Instituto Nacional de Investigaciones Nucleares, Carretera México-Toluca s/n, La Marquesa, Ocoyoacac, Edo. de México, C.P. 52750, Mexico.
Instituto Nacional de Investigaciones Nucleares, Carretera México-Toluca s/n, La Marquesa, Ocoyoacac, Edo. de México, C.P. 52750, Mexico.
Environ Toxicol Pharmacol. 2020 Nov;80:103464. doi: 10.1016/j.etap.2020.103464. Epub 2020 Aug 1.
The identification of substances that prevent or minimize the detrimental effects of ionizing radiation is an essential undertaking. The aim of this paper was to evaluate and compare the radioprotective potential of chlorophyllin, protoporphyrin and bilirubin, with amifostine®, an US Food & Drug Administration approved radioprotector Using the somatic mutation and recombination assay in the Drosophila melanogaster wing, it was found that pretreatment (1-9 h) with any of the porphyrins or amifostine® alone, did not affect the larva-adult viability or the basal frequency of mutation. However, they were associated with significant reductions in frequency of somatic mutation and recombination compared with the gamma-irradiated (20 Gy) control as follows: bilirubin (69.3 %)> chlorophyllin (40.0 %)> protoporphyrin (39.0 %)> amifostine® (19.7 %). Bilirubin also caused a 16 % increase in larva-adult viability with 3 h of pretreatment respect to percentage induced in 20 Gy control group. Whilst amifostine® was associated with lower genetic damage after pre-treatment of 1 and 3 h, this did not attain significance. These findings suggest that the tested porphyrins may have some potential as radioprotectant agents.
确定能够预防或减轻电离辐射有害影响的物质是一项至关重要的任务。本文旨在评估和比较叶绿酸、原卟啉和胆红素与美国食品和药物管理局批准的放射保护剂氨磷汀的放射防护潜力。利用黑腹果蝇翅的体突变和重组检测，发现单独用任何一种卟啉或氨磷汀预处理（1-9 小时），不会影响幼虫-成虫的存活率或突变的基础频率。然而，与γ射线照射（20Gy）对照组相比，它们与体细胞突变和重组频率的显著降低有关，具体如下：胆红素（69.3%）>叶绿酸（40.0%）>原卟啉（39.0%）>氨磷汀（19.7%）。胆红素在预处理 3 小时后还使幼虫-成虫的存活率增加了 16%，与 20Gy 对照组诱导的百分比相比。虽然氨磷汀在预处理 1 小时和 3 小时后与较低的遗传损伤相关，但这并不显著。这些发现表明，所测试的卟啉类物质可能具有一定的放射保护剂潜力。
