Nelson Matthew N, Jabbari Jafar S, Turakulov Rust, Pradhan Aneeta, Pazos-Navarro Maria, Stai Jacob S, Cannon Steven B, Real Daniel
CSIRO Agriculture & Food, Floreat, WA 6014, Australia.
The UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia.
Plants (Basel). 2020 Jul 31;9(8):973. doi: 10.3390/plants9080973.
We present the first genetic map of tedera ( (L.) C.H. Stirton), a drought-tolerant forage legume from the Canary Islands with useful pharmaceutical properties. It is also the first genetic map for any species in the tribe Psoraleeae (Fabaceae). The map comprises 2042 genotyping-by-sequencing (GBS) markers distributed across 10 linkage groups, consistent with the haploid chromosome count for this species ( = 10). Sequence tags from the markers were used to find homologous matches in the genome sequences of the closely related species in the Phaseoleae tribe: soybean, common bean, and cowpea. No tedera linkage groups align in their entirety to chromosomes in any of these phaseoloid species, but there are long stretches of collinearity that could be used in tedera research for gene discovery purposes using the better-resourced phaseoloid species. Using Ks analysis of a tedera transcriptome against five legume genomes provides an estimated divergence time of 17.4 million years between tedera and soybean. Genomic information and resources developed here will be invaluable for breeding tedera varieties for forage and pharmaceutical purposes.
我们展示了泰德拉草（(L.) C.H. Stirton）的首张遗传图谱，泰德拉草是一种来自加那利群岛的耐旱豆科牧草，具有有用的药用特性。这也是补骨脂族（豆科）中任何物种的首张遗传图谱。该图谱包含2042个通过测序进行基因分型（GBS）的标记，分布在10个连锁群上，与该物种的单倍体染色体数（n = 10）一致。标记的序列标签用于在菜豆族的近缘物种（大豆、普通菜豆和豇豆）的基因组序列中寻找同源匹配。泰德拉草的连锁群没有一个与这些菜豆属物种的染色体完全对齐，但存在大片共线性区域，可用于泰德拉草研究，以便利用资源更丰富的菜豆属物种进行基因发现。通过对泰德拉草转录组与五个豆科植物基因组进行Ks分析，估计泰德拉草与大豆之间的分化时间为1740万年。这里开发的基因组信息和资源对于培育用于饲料和药用目的的泰德拉草品种将具有极高价值。
