Kroc Magdalena, Koczyk Grzegorz, Święcicki Wojciech, Kilian Andrzej, Nelson Matthew N
Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479, Poznan, Poland.
Theor Appl Genet. 2014 May;127(5):1237-49. doi: 10.1007/s00122-014-2294-y. Epub 2014 Mar 15.
This is the first clear evidence of duplication and/or triplication of large chromosomal regions in a genome of a Genistoid legume, the most basal clade of Papilionoid legumes. Lupinus angustifolius L. (narrow-leafed lupin) is the most widely cultivated species of Genistoid legume, grown for its high-protein grain. As a member of this most basal clade of Papilionoid legumes, L. angustifolius serves as a useful model for exploring legume genome evolution. Here, we report an improved reference genetic map of L. angustifolius comprising 1207 loci, including 299 newly developed Diversity Arrays Technology markers and 54 new gene-based PCR markers. A comparison between the L. angustifolius and Medicago truncatula genomes was performed using 394 sequence-tagged site markers acting as bridging points between the two genomes. The improved L. angustifolius genetic map, the updated M. truncatula genome assembly and the increased number of bridging points between the genomes together substantially enhanced the resolution of synteny and chromosomal colinearity between these genomes compared to previous reports. While a high degree of syntenic fragmentation was observed that was consistent with the large evolutionary distance between the L. angustifolius and M. truncatula genomes, there were striking examples of conserved colinearity of loci between these genomes. Compelling evidence was found of large-scale duplication and/or triplication in the L. angustifolius genome, consistent with one or more ancestral polyploidy events.
这是首次在蝶形花科最基部的分支——金雀花亚科豆科植物的基因组中发现大染色体区域重复和/或三倍化的确切证据。窄叶羽扇豆（Lupinus angustifolius L.）是种植最广泛的金雀花亚科豆科植物，因其高蛋白籽粒而被种植。作为蝶形花科最基部这一分支的成员，窄叶羽扇豆是探索豆科植物基因组进化的有用模型。在此，我们报告了一份改进的窄叶羽扇豆参考遗传图谱，该图谱包含1207个位点，其中包括299个新开发的多样性阵列技术标记和54个新的基于基因的PCR标记。利用394个序列标签位点标记作为两个基因组之间的桥梁点，对窄叶羽扇豆和蒺藜苜蓿的基因组进行了比较。与之前的报告相比，改进后的窄叶羽扇豆遗传图谱、更新后的蒺藜苜蓿基因组组装以及基因组之间桥梁点数量的增加，显著提高了这些基因组之间的同线性和染色体共线性分辨率。虽然观察到高度的同线性片段化，这与窄叶羽扇豆和蒺藜苜蓿基因组之间较大的进化距离一致，但在这些基因组之间存在着显著的位点保守共线性实例。在窄叶羽扇豆基因组中发现了大规模重复和/或三倍化的确凿证据，这与一次或多次祖先多倍体事件一致。
