Department of Biotechnology, Assam University, Silchar, India.
Department of Zoology, M. H. C. M. Science College, Hailakandi, India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2020 Dec;31(8):313-326. doi: 10.1080/24701394.2020.1800661. Epub 2020 Aug 5.
Analysis of codon usage bias (CUB) is very much important in perceiving the knowledge of molecular biology, the discovery of a new gene, designing of transgenes and evolution of gene. In this study, we analyzed compositional features and codon usage of (, and ) genes among the classes of Arthropoda to explore the pattern of CUB as no research work was reported yet. Nucleotide composition analysis in genes suggested that the genes were AT-rich in all the four classes of Arthropoda. CUB was low in all the classes of Arthropoda for genes as revealed from a high effective number of codons (ENC). We also found that the evolutionary forces namely mutation pressure and natural selection were the key influencing factors in CUB among genes as revealed by correlation analysis between overall nucleotide composition and nucleotide composition at the 3rd codon position. Correspondence analysis suggested that the pattern of CUB was different among the classes of Arthropoda. Further, it was revealed from the neutrality plot that natural selection had a dominant role while mutation pressure exhibited a minor role in structuring the pattern of codon usage in all the classes of Arthropoda across , and genes.
分析密码子使用偏性（CUB）对于理解分子生物学知识、发现新基因、设计转基因和基因进化非常重要。在这项研究中，我们分析了节肢动物纲中（、和）基因的组成特征和密码子使用情况，以探索 CUB 的模式，因为目前还没有研究报道过。在所有 4 个节肢动物纲中，基因的核苷酸组成分析表明这些基因富含 AT。所有节肢动物纲的基因的 CUB 都较低，这从高有效密码子数（ENC）中可以看出。我们还通过整体核苷酸组成与第 3 位密码子位置核苷酸组成之间的相关性分析发现，进化压力即突变压力和自然选择是影响基因 CUB 的关键因素。对应分析表明，CUB 的模式在节肢动物纲之间存在差异。此外，从中性图中可以看出，在所有节肢动物纲中，自然选择在构建 、和 基因的密码子使用模式方面发挥着主导作用，而突变压力则起着次要作用。
