Senior B W, Hughes C
Department of Medical Microbiology, Dundee University Medical School, Ninewells Hospital.
J Med Microbiol. 1988 Jan;25(1):17-25. doi: 10.1099/00222615-25-1-17.
A collection of 198 clinical isolates of strains belonging to the tribe Proteeae was examined for haemolytic activity on blood agar and in Brain Heart Infusion Broth. The strains were of diverse bacteriocin and O-antigenic types and from a wide variety of sources. They included representatives of all species of Morganella, Proteus and Providencia. Approximately half of the M. morgani strains were haemolytic on blood agar. This activity was not associated with any particular bacteriocin type. The haemolysin was also produced during exponential growth in broth and was thermolabile and calcium dependent. All P. mirabilis strains and some P. vulgaris strains were non-haemolytic on blood agar. However, most strains of the Proteus spp., irrespective of their bacteriocin and antigenic type, produced, over a short period during exponential growth in broth, a heat-stable, cell-associated calcium-independent haemolysin. A smaller proportion of P. vulgaris and P. penneri strains produced, in addition, a thermolabile, calcium-dependent haemolysin which was associated with the formation of large haemolytic zones on blood agar. The relationship of these haemolysins to Escherichia coli haemolysin and their possible role in virulence is discussed. Haemolysin production was not found in any of the 74 strains of four species of Providencia.
对198株变形杆菌族菌株的临床分离株进行了检测，观察其在血琼脂平板和脑心浸液肉汤中的溶血活性。这些菌株具有不同的细菌素和O抗原类型，来源广泛。它们包括摩根菌属、变形杆菌属和普罗威登斯菌属所有物种的代表菌株。大约一半的摩根摩根菌菌株在血琼脂平板上具有溶血活性。这种活性与任何特定的细菌素类型无关。溶血素在肉汤中的指数生长期也会产生，且具有热不稳定性和钙依赖性。所有奇异变形杆菌菌株和一些普通变形杆菌菌株在血琼脂平板上不溶血。然而，大多数变形杆菌属菌株，无论其细菌素和抗原类型如何，在肉汤中的指数生长期短时间内会产生一种热稳定的、与细胞相关的非钙依赖性溶血素。此外，较小比例的普通变形杆菌和彭氏变形杆菌菌株还产生一种热不稳定的、钙依赖性溶血素，这与血琼脂平板上大溶血圈的形成有关。讨论了这些溶血素与大肠杆菌溶血素的关系及其在毒力中的可能作用。在74株普罗威登斯菌属的四个物种中，均未发现溶血素产生。
