Characterization of cell-bound and cell-free hemolytic activity of Proteus strains.

The relationship between growth condition and production of cell-bound hemolysin by Proteus mirabilis S1959 strain was investigated. Hemolytic activity was not dependent on type of medium, oxygen accessibility and was inhibited in presence of N-ethylmaleimide, different sera or trypsine. Cell-free hemolysin was released to the medium during stationary-death phases of growth of fluid Proteus mirabilis and vulgaris cultures.