Williams D L, Sherwood E R, Browder I W, McNamee R B, Jones E L, Rakinic J, Di Luzio N R
Department of Physiology, Tulane University, School of Medicine, New Orleans, Louisiana 70112.
J Surg Res. 1988 Jan;44(1):54-61. doi: 10.1016/0022-4804(88)90122-9.
Previous studies from our laboratory have demonstrated that glucan, a nonspecific immunomodulator, modifies the course of murine Escherichia coli peritonitis. The protective effect of glucan was mediated, in part, by macrophages. In the present study, leukocyte dynamics in the peritoneal cavity and peripheral blood of glucan-treated mice following E. coli challenge was examined. Additional studies examined in vitro bone marrow proliferation, as well as phagocytosis and intracellular killing of E. coli by neutrophils following glucan administration. ICR/HSD mice were injected ip with glucan (150 mg/kg) or dextrose (5% w/v) on Days 5 and 3 prior to ip challenge with 1 X 10(8) E. coli. Glucan increased (P less than 0.05) total peritoneal neutrophil numbers prior to and following septic challenge. Examination of peripheral blood revealed that ip glucan treatment in E. coli peritonitis significantly (P less than 0.001) increased the number of circulating neutrophils. Additionally, neutrophils from glucan-treated mice showed increased phagocytosis of E. coli in vitro. Glucan therapy also increased bone marrow proliferation. We conclude that (1) glucan enhances peritoneal neutrophil levels, (2) peripheral blood neutrophils are increased following glucan and E. coli, (3) ip glucan increases bone marrow proliferation, and (4) neutrophils from glucan-treated mice showed enhanced phagocytosis of E. coli in vitro. Thus, the beneficial effect of glucan is mediated not only by activated macrophages, but also by the neutrophilic leukocyte.
我们实验室之前的研究表明，葡聚糖作为一种非特异性免疫调节剂，可改变小鼠大肠杆菌性腹膜炎的病程。葡聚糖的保护作用部分是由巨噬细胞介导的。在本研究中，检测了葡聚糖处理的小鼠在受到大肠杆菌攻击后腹腔和外周血中的白细胞动态变化。另外的研究检测了体外骨髓增殖情况，以及给予葡聚糖后中性粒细胞对大肠杆菌的吞噬作用和细胞内杀伤作用。在腹腔注射1×10(8) 大肠杆菌进行攻击前3天和5天，给ICR/HSD小鼠腹腔注射葡聚糖（150毫克/千克）或葡萄糖（5% w/v）。葡聚糖使脓毒症攻击前后的腹腔总中性粒细胞数量增加（P<0.05）。对外周血的检测显示，在大肠杆菌性腹膜炎中腹腔注射葡聚糖治疗可显著（P<0.001）增加循环中性粒细胞的数量。此外，来自葡聚糖处理小鼠的中性粒细胞在体外对大肠杆菌的吞噬作用增强。葡聚糖治疗还增加了骨髓增殖。我们得出结论：（1）葡聚糖可提高腹腔中性粒细胞水平；（2）葡聚糖和大肠杆菌攻击后外周血中性粒细胞增加；（3）腹腔注射葡聚糖可增加骨髓增殖；（4）来自葡聚糖处理小鼠的中性粒细胞在体外对大肠杆菌的吞噬作用增强。因此，葡聚糖有益作用不仅由活化的巨噬细胞介导，也由嗜中性白细胞介导。
