Department of Bioprocess Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, Ul. Wolczanska 213, 90-924 Lodz, Poland.
Department of Bioprocess Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, Ul. Wolczanska 213, 90-924 Lodz, Poland.
J Biosci Bioeng. 2020 Nov;130(5):503-513. doi: 10.1016/j.jbiosc.2020.06.012. Epub 2020 Aug 2.
The production of secondary metabolites in the submerged co-cultures of Penicillium rubens Wisconsin 54-1255 and Aspergillus terreus ATCC 20542 was evaluated. The biosynthetic capabilities of the two strains were compared in a set of diverse liquid media that differed with respect to the initial levels of glucose, lactose and yeast extract, contained carrot juice or vegetable/turkey puree as additional nutrient sources or were supplemented with phenylacetic acid, the side-chain precursor of penicillin G. The main goal of the study was to investigate the interactions between A. terreus and P. rubens that might contribute to the changes of secondary metabolite titers. Briefly, the biosynthesis of octaketide metabolites (+)-geodin and asterric acid was visibly enhanced as a result of replacing the conventional monocultures with the co-culture systems, but solely in the media containing not more than 5 g L of yeast extract. By contrast, no marked enhancement was observed with respect to the biosynthesis of penicillin G, lovastatin, chrysogine, 4a,5-dihydromevinolinic acid and 3α-hydroxy-3,5-dihydromonacolin L acid. It was shown that the relationships between medium composition and product titers were clearly different in monoculture variants than in the corresponding co-cultures. Finally, it was demonstrated that the utilization of penicillin precursors by P. rubens can be blocked under the conditions of co-cultivation.
红曲霉 Wisconsin 54-1255 和土曲霉 ATCC 20542 的共培养物中次生代谢产物的生产情况进行了评估。在一系列不同的液体培养基中比较了两种菌株的生物合成能力，这些培养基在葡萄糖、乳糖和酵母提取物的初始水平、含有胡萝卜汁或蔬菜/火鸡泥作为额外的营养源或添加苯乙酸（青霉素 G 的侧链前体）方面存在差异。研究的主要目的是研究土曲霉和红曲霉之间的相互作用，这些相互作用可能导致次生代谢产物产量的变化。简而言之，与传统的单培养物相比，共培养物系统明显促进了八酮代谢物 (+)-geodin 和 asterric acid 的生物合成，但仅在含有不超过 5 g/L 酵母提取物的培养基中。相比之下，青霉素 G、洛伐他汀、chrysogine、4a,5-二氢麦角甾酮酸和 3α-羟基-3,5-二氢麦角甾醇 L 酸的生物合成没有明显增强。结果表明，在单培养物变体中，培养基组成与产物产量之间的关系明显不同于相应的共培养物。最后，证明在共培养条件下，红曲霉可以阻止青霉素前体的利用。
