Hollis G F, Gazdar A F, Bertness V, Kirsch I R
National Cancer Institute-Navy Medical Oncology Branch, Bethesda, Maryland 20814.
Mol Cell Biol. 1988 Jan;8(1):124-9. doi: 10.1128/mcb.8.1.124-129.1988.
A complex translocation has interrupted the third exon of the c-myc gene in human plasma cell myeloma tumor cells and a derivative cell line (NCI-H929). As a result of this rearrangement, a chimeric mRNA is expressed which commences 5' of the c-myc coding region and includes sequences introduced by the translocation event. All of the detectable c-myc-containing mRNA in the tumor and cell line was derived from this rearranged c-myc allele. This chimeric c-myc mRNA, in which most of the germ line c-myc 3' untranslated region has been replaced, was greater than sevenfold more stable than c-myc transcripts with intact 3' ends. This suggests that the 3' untranslated region may play an important role in c-myc mRNA stability.
在人浆细胞骨髓瘤肿瘤细胞和一个衍生细胞系（NCI-H929）中，一个复杂易位中断了c-myc基因的第三个外显子。由于这种重排，一种嵌合mRNA得以表达，该mRNA起始于c-myc编码区的5'端，并包含由易位事件引入的序列。肿瘤和细胞系中所有可检测到的含c-myc的mRNA均源自这个重排的c-myc等位基因。这种嵌合c-myc mRNA，其中大部分种系c-myc 3'非翻译区已被取代，其稳定性比具有完整3'端的c-myc转录本高七倍以上。这表明3'非翻译区可能在c-myc mRNA稳定性中起重要作用。
