采用质谱法检测严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）。
Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Mass Spectrometry.
机构信息
Center for Histology, Cytology and Molecular Diagnostics Trier, 54296 Trier, Germany.
Department of Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, Germany.
出版信息
Viruses. 2020 Aug 4;12(8):849. doi: 10.3390/v12080849.
BACKGROUND
Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS).
METHODS
Viral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared.
RESULTS
Results of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays.
CONCLUSIONS
MS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs.
背景
实时逆转录聚合酶链反应（rRT-PCR）扩增病毒核糖核酸（RNA）是检测严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的金标准。自最初爆发以来，检测和隔离患者的策略对于避免病毒的失控传播非常重要。尽管检测能力已经扩大，但仍需要可靠的高通量测试系统，特别是那些需要替代耗材的系统。因此，我们测试并比较了两种用于检测病毒 PCR 产物的不同方法：rRT-PCR 和质谱法（MS）。
方法
从口咽或鼻咽拭子中分离和扩增病毒 RNA。共分析了 22 份 rRT-PCR 检测 SARS-CoV-2 阳性和 22 份阴性的样本。比较了 rRT-PCR 和 MS 方案的结果。
结果
rRT-PCR 和 MS 测试系统在所有样本中的结果均一致。rRT-PCR 的检测结果更快。两种检测方法的实际操作时间相当。
结论
MS 是一种快速、可靠且具有成本效益的替代方法，可用于从口腔和鼻咽拭子中检测 SARS-CoV-2。
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