Sivanesan Iyyakkannu, Gopal Judy, Surya Vinay Rohit, Luke Elizabeth Hanna, Oh Jae-Wook, Muthu Manikandan
Department of Bioresources and Food Science, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul, 05029, South Korea.
Tagore Medical College and Hospital, Rathinamangalam, Melakottaiyur, Chennai, Tamil Nadu, 600127, India.
Trends Analyt Chem. 2022 May;150:116569. doi: 10.1016/j.trac.2022.116569. Epub 2022 Feb 22.
MALDI-TOF-MS has essentially delivered more than expected with respect to clinical pathogens. Viruses are the most versatile entities of clinical pathogens that have challenged well-established microbiological methodologies. This review evaluates the existing scenario with respect to MALDI TOF-MS analytical technique in the successful analysis of viral pathogens. The milestones achieved with respect to detection and identification of COVID-19 has been presented. The fact that only a handful of scattered applications for COVID-19 exist has been pointed out in the review. Further, the lapses in the utilization of the available state-of-the art MALDI-TOF-MS variants/benchmark sophistications for COVID-19 analysis, are highlighted. When the world is seeking for rapid solutions for early, sensitive, rapid COVID-19 diagnosis, maybe MALDI-TOF-MS, may be the actual 'gold standard'. Reverting to the title, this review emphasizes that there is a need for extrapolating MALDI-TOF-MS for COVID-19 analysis and this calls for urgent scientific attention.
就临床病原体而言，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）所取得的成果基本上超出了预期。病毒是临床病原体中最为多样的实体，对成熟的微生物学方法构成了挑战。本综述评估了MALDI-TOF-MS分析技术在成功分析病毒病原体方面的现有情况。文中介绍了在新冠病毒（COVID-19）检测和鉴定方面所取得的里程碑式进展。该综述指出，目前针对COVID-19的应用仅有少数分散的案例。此外，还强调了在利用现有最先进的MALDI-TOF-MS变体/基准技术进行COVID-19分析时存在的不足。当全世界都在寻求针对COVID-19进行早期、灵敏、快速诊断的快速解决方案时，也许MALDI-TOF-MS可能才是真正的“金标准”。回到标题，本综述强调需要将MALDI-TOF-MS推广用于COVID-19分析，这需要科学界给予迫切关注。
