Owusu-Darko Rodney, Allam Mushal, Ismail Arshad, Ferreira Carlos A S, Oliveira Sílvia D de, Buys Elna M
Department of Consumer and Food Sciences, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.
National Institute for Communicable Diseases, Private Bag X4, Sandringham, Johannesburg 2131, South Africa.
Microorganisms. 2020 Aug 4;8(8):1185. doi: 10.3390/microorganisms8081185.
currently possesses one of the most highly heat-resistant spores (HRS), which can withstand ultra-high temperature (UHT) processing. Determination of multiple whole genome sequences of . provided an opportunity to perform the first comparative genome analysis between strains and with . , . , and . groups. In this study, five whole genome sequences of strains, including those belonging to the HRS clone (SAD and BR12) normally isolated from UHT milk, were compared with the aforementioned species for gene clusters responsible for heat resistance. In the phylogenomic analysis, , with its closest phylogenetic neighbor, . , clustered with . and . Heat shock proteins GrpE, GroES, GroEL, and DnaK presented identical sequences for all . strains, indicating that differences in functional efficiency are not involved in the thermal resistance variations. However, comparing all species evaluated, . exhibited a different gene configuration in the chromosomal region of the heat shock protein GrpE. Furthermore, only . strains presented the stage II sporulation protein P gene located in this region. Multisequence alignment and phylogenetic analysis of the ClpB protein showed differences for HRS and non-HRS strains. The study identified ClpC, ClpE, and ClpX as the three ATPases putatively involved in protein disaggregation in . . exhibits high homology with other species in the DnaK, DnaJ, GroEL, and GroES cluster of genes involved in heat resistance. The data presented here pave the way to select and evaluate the phenotypic effects of genes putatively involved in heat resistance.
目前拥有一种耐热性极高的孢子（HRS），它能够承受超高温（UHT）处理。对……的多个全基因组序列进行测定，为首次在菌株之间以及与……、……和……组进行比较基因组分析提供了机会。在本研究中，将5个……菌株的全基因组序列，包括那些属于通常从超高温灭菌牛奶中分离出的HRS克隆（SAD和BR12）的序列，与上述……物种进行比较，以寻找负责耐热性的基因簇。在系统发育基因组分析中，……与其最接近的系统发育邻居……，与……和……聚集在一起。热休克蛋白GrpE、GroES、GroEL和DnaK在所有……菌株中呈现相同序列，表明功能效率的差异与耐热性变化无关。然而，在比较所有评估的物种时，……在热休克蛋白GrpE的染色体区域表现出不同的基因构型。此外，只有……菌株在该区域呈现出II期芽孢形成蛋白P基因。ClpB蛋白的多序列比对和系统发育分析显示HRS和非HRS菌株存在差异。该研究确定ClpC、ClpE和ClpX为……中可能参与蛋白质解聚的三种ATP酶。……在参与耐热性的DnaK、DnaJ、GroEL和GroES基因簇中与其他……物种具有高度同源性。此处呈现的数据为选择和评估可能参与耐热性的基因的表型效应铺平了道路。
