Kinlough-Rathbone R L, Mustard J F, Packham M A, Perry D W, Reimers H J, Cazenave J P
Thromb Haemost. 1977 Apr 30;37(2):291-308.
We have shown previously that washed human platelets resuspended in Tyrode solution containing albumin and apyrase maintain their disc shape and their ability to aggregate upon the addition of low concentration of ADP, providing fibrinogen is added to the suspending medium. We have now examined their responses to other aggregating and release-inducing agents. Collagen, arachidonate, thrombin, immune serum globulin, the ionophore A23, 187 and phytohaemagglutinin from Phaseolus vulgaris caused aggregation and release of granule contents. The response to adrenaline was variable. Serotonin caused the platelets to change shape but no aggregation or release occurred. Addition of a small amount of plasma was necessary for ristocetin-induced aggregation. Polylysine caused immediate platelet-to-platelet adherence with little or no release of granule contents. Responses to collagen or thrombin were greater in a modified medium containing magnesium but no calcium; in this medium, aggregation caused by ADP or polylysine was followed by the release of granule contents whereas these agents caused aggregation without release in a medium with both calcium and magnesium. When protein was omitted from the suspending medium, platelet aggregation in response to ADP was variable. In this medium, collagen and thrombin caused more extensive release than in the albumin-containing medium. Aggregation by polylysine was accompanied by release and extensive lysis in the protein-free medium. Thus, the composition of the final resuspending medium has a major effect on the responses of washed human platelets to aggregating agents.
我们之前已经表明，悬浮于含有白蛋白和腺苷三磷酸双磷酸酶的台氏液中的洗涤过的人血小板，在添加低浓度二磷酸腺苷（ADP）时，只要在悬浮介质中添加纤维蛋白原，就能保持其圆盘形状以及聚集能力。我们现在研究了它们对其他聚集和诱导释放剂的反应。胶原蛋白、花生四烯酸、凝血酶、免疫血清球蛋白、离子载体A23187以及菜豆中的植物血凝素会引起聚集并释放颗粒内容物。对肾上腺素的反应则各不相同。血清素会使血小板改变形状，但不会发生聚集或释放。瑞斯托菌素诱导的聚集需要添加少量血浆。聚赖氨酸会导致血小板立即相互黏附，颗粒内容物很少释放或不释放。在含有镁但不含钙的改良培养基中，对胶原蛋白或凝血酶的反应更强；在这种培养基中，由ADP或聚赖氨酸引起的聚集之后会释放颗粒内容物，而在同时含有钙和镁的培养基中，这些试剂会引起聚集但不释放。当悬浮介质中不含蛋白质时，血小板对ADP的聚集反应各不相同。在这种培养基中，胶原蛋白和凝血酶引起的释放比在含白蛋白的培养基中更广泛。在无蛋白培养基中，聚赖氨酸引起的聚集伴随着释放和广泛的溶解。因此，最终悬浮介质的组成对洗涤过的人血小板对聚集剂的反应有重大影响。
