College of Biology and Food Engineering, Anyang Institute of Technology, Anyang, Henan 455000, PR China.
Anyang Academy of Agriculture Sciences, Anyang, Henan 455000, PR China.
Genomics. 2020 Nov;112(6):4463-4473. doi: 10.1016/j.ygeno.2020.07.045. Epub 2020 Aug 5.
Long non-coding RNAs (lncRNAs) play an important function in plant growth and development as well as response to stresses. However, little information was known in foxtail millet; no study was reported on lncRNAs in plant response to herbicide treatment. In this study, by using deep sequencing and advanced bioinformatic analysis, a total of 2547 lncRNAs were identified, including 787 known and 1760 novel lncRNAs. These lncRNAs are distributed across all 9 chromosomes, and the majority were located in the intergenic region with 1-2 exons. These lncRNAs were differentially expressed between different genotypes under different herbicide treatments. lncRNAs regulate plant growth and development as well as response to herbicide treatments through targeting protein-coding genes that directly relate to chemical metabolism and defense system. Multiple potential target genes and lncRNA-mRNA-miRNA gene networks were discovered. These results elucidate the potential roles of lncRNAs in plant response to herbicides.
长非编码 RNA（lncRNAs）在植物生长发育以及对胁迫的反应中发挥着重要作用。然而，关于谷子中的 lncRNAs 知之甚少；尚未有研究报道 lncRNAs 在植物对除草剂处理的反应中的作用。在这项研究中，通过使用深度测序和先进的生物信息学分析，共鉴定出 2547 个 lncRNAs，包括 787 个已知和 1760 个新的 lncRNAs。这些 lncRNAs 分布在所有 9 条染色体上，大多数位于基因间区域，具有 1-2 个外显子。这些 lncRNAs 在不同除草剂处理下不同基因型之间的表达存在差异。lncRNAs 通过靶向与化学代谢和防御系统直接相关的蛋白质编码基因来调节植物的生长发育以及对除草剂的反应。发现了多个潜在的靶基因和 lncRNA-mRNA-miRNA 基因网络。这些结果阐明了 lncRNAs 在植物对除草剂反应中的潜在作用。
