Department of Oral Surgery and Pathology, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, CEP: 31270-901, Brazil.
Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, CEP: 31270-901, Brazil.
Arch Oral Biol. 2020 Oct;118:104856. doi: 10.1016/j.archoralbio.2020.104856. Epub 2020 Aug 1.
Despite the high frequency of impacted teeth and increased frequency of lesions in dental follicles (DF) with aging, DF age-changes remain unclear. We compared the global methylation and hydroxymethylation profiles in DF in relation to age.
DF associated with impacted lower third molars were obtained from 59 individuals. Global DNA methylation (5mC content) and hydroxymethylation (5hmC) were evaluated by ELISA. We tested the correlation between 5mC and 5hmC content, and the correlation of each with patients' age. The differences in age, 5mC, and 5hmC in DF from men/women, and location (left/right mandible) was tested.
The mean age of the 59 individuals was 19.56 ± 3.92, ranging from 13 to 31 years, and most were women (n = 39). 5hmC content and age up to 19 years were inversely correlated (Spearman's correlation coefficient=-0.552, p = 0.0003, n = 38). There was no relationship between 5hmC and 5mC content. There was no difference in the medians of age (p = 0.25), 5hmC (p = 0.33) and 5mC (p = 0.86) between men/women, nor in the medians of age (p = 0.39), 5hmC (p = 0.99) and 5mC (p = 0.22) between the left/right side of the tooth extraction.
An inverse correlation between 5hmC and age was established, with no correlation between 5mC and 5hmC content in DF. The biological meaning of such a decrease of global DNA hydroxymethylation with age in DF remains to be clarified.
尽管随着年龄的增长，牙齿受影响的频率增加，牙囊（DF）中的病变频率也增加，但 DF 的年龄变化仍不清楚。我们比较了与年龄相关的 DF 中的整体甲基化和羟甲基化谱。
从 59 名个体中获得与下颌第三磨牙受影响相关的 DF。通过 ELISA 评估全局 DNA 甲基化（5mC 含量）和羟甲基化（5hmC）。我们测试了 5mC 和 5hmC 含量之间的相关性，以及它们与患者年龄的相关性。测试了来自男性/女性的 DF 中年龄、5mC 和 5hmC 的差异，以及位置（左右下颌骨）的差异。
59 名个体的平均年龄为 19.56±3.92 岁，年龄范围为 13 至 31 岁，其中大多数为女性（n=39）。5hmC 含量与 19 岁以下年龄呈负相关（Spearman 相关系数=-0.552，p=0.0003，n=38）。5hmC 与 5mC 含量之间没有关系。男性/女性之间的年龄中位数（p=0.25）、5hmC 中位数（p=0.33）和 5mC 中位数（p=0.86）无差异，牙齿提取的左侧/右侧之间的年龄中位数（p=0.39）、5hmC 中位数（p=0.99）和 5mC 中位数（p=0.22）也无差异。
建立了 5hmC 与年龄之间的负相关关系，DF 中 5mC 与 5hmC 含量之间无相关性。DF 中随着年龄增长而降低的整体 DNA 羟甲基化的生物学意义仍有待阐明。
