State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
City of Hope National Medical Center, Duarte, CA, USA.
Food Chem. 2021 Jan 30;336:127718. doi: 10.1016/j.foodchem.2020.127718. Epub 2020 Aug 1.
A novel dual near-infrared fluorescence-based lateral flow immunosensor was developed to determine zearalenone and deoxynivalenol in maize. Two near-infrared dyes with distinct fluorescence characteristics were utilized to separately label the anti-zearalenone and anti-deoxynivalenol antibodies as detection reagents. The capture antigens zearalenone-BSA and deoxynivalenol-BSA were mixed and immobilized on the same test line of nitrocellulose membrane. This assay format facilitates simultaneous detection of the two mycotoxins on a single test line. After optimizing experimental parameters, the limits of detection for zearalenone and deoxynivalenol were as low as 0.55 μg/kg and 3.8 μg/kg in maize, respectively. The spiking experiment yielded recovery ratios ranging from 81.7% to 107.3% with coefficients of variation less than 14% demonstrating high assay accuracy and precision. Moreover, the actual sample analysis produced consistent results between this method and instrumental method. Therefore, the developed immunosensor can serve as an accurate and efficient approach for monitoring mycotoxins in agricultural products.
一种新型的基于双近红外荧光的侧向流动免疫传感器被开发出来，用于检测玉米中的玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇。两种具有不同荧光特性的近红外染料被分别标记为抗玉米赤霉烯酮和抗脱氧雪腐镰刀菌烯醇抗体作为检测试剂。将玉米赤霉烯酮-BSA 和脱氧雪腐镰刀菌烯醇-BSA 作为捕获抗原混合并固定在硝酸纤维素膜的同一测试线上。这种检测模式可以在单个测试线上同时检测两种真菌毒素。在优化实验参数后，该方法对玉米中玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇的检出限分别低至 0.55μg/kg 和 3.8μg/kg。加标实验的回收率在 81.7%至 107.3%之间，变异系数小于 14%，表明该方法具有较高的准确性和精密度。此外，实际样品分析结果与仪器法之间具有一致性。因此，该免疫传感器可作为一种准确、高效的方法用于监测农产品中的真菌毒素。
