Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
Merden Dental Hospital, Bucheon-si 14544, Korea.
Medicina (Kaunas). 2020 Aug 5;56(8):389. doi: 10.3390/medicina56080389.
(Noni) has been widely used in herbal remedies to treat and prevent various kinds of diseases. We conducted this study to evaluate the effects of Noni extract on the maintenance of morphology, the improvement of cellular viability, and the enhancement of osteogenesis of stem cell spheroids. We cultured stem cell spheroids made with gingiva-derived stem cells in the presence of Noni extract at concentrations of 10, 100 and 200 ng/mL. We performed analysis of the cell morphology and changes in the cellular viability. We conducted alkaline phosphatase activity assays using a kit, and mineralization assays using an anthraquinone dye to evaluate the osteogenesis of stem cell spheroids with the addition of Noni extract. The applied cells formed spheroids well, and the addition of Noni at 10, 100 and 200 ng/mL concentrations did not produce significant morphological changes. The quantitative values for cellular viability on Day 3 showed that the absorbance values at 450 nm were 0.314 ± 0.013, 0.318 ± 0.008, 0.304 ± 0.000 and 0.300 ± 0.011 for Noni at 0, 10, 100 and 200 ng/mL concentrations, respectively. The results of alkaline phosphatase activity with absorbance values at 405 nm were 0.189 ± 0.019, 0.174 ± 0.023, 0.192 ± 0.014 and 0.210 ± 0.062 for Noni at 0, 10, 100 and 200 ng/mL concentrations, respectively, on Day 4. There were significantly higher values of Alizarin Red S staining for Noni in the 10, 100 and 200 ng/mL groups, with the highest value at 100 ng/mL when compared with the unloaded control on Day 14. Based on these findings, we concluded that Noni extract might be applied for the enhanced osteogenic differentiation of stem cell spheroids.
（诺丽果）已广泛用于草药疗法中，以治疗和预防各种疾病。我们进行这项研究是为了评估诺丽果提取物对维持细胞形态、提高细胞活力和增强干细胞球成骨的影响。我们在浓度为 10、100 和 200ng/ml 的诺丽果提取物存在下培养由牙龈来源的干细胞制成的干细胞球。我们进行了细胞形态分析和细胞活力变化分析。我们使用试剂盒进行碱性磷酸酶活性测定，并使用蒽醌染料进行矿化测定，以评估添加诺丽果提取物后干细胞球的成骨作用。应用的细胞很好地形成了球状体，添加 10、100 和 200ng/ml 浓度的诺丽果没有产生明显的形态变化。第 3 天细胞活力的定量值显示，450nm 处的吸光度值分别为 0.314±0.013、0.318±0.008、0.304±0.000 和 0.300±0.011，对于 0、10、100 和 200ng/ml 浓度的诺丽果，分别为 0.189±0.019、0.174±0.023、0.192±0.014 和 0.210±0.062，在第 4 天，用 405nm 处的吸光度值进行碱性磷酸酶活性测定，对于 0、10、100 和 200ng/ml 浓度的诺丽果，分别为 0.189±0.019、0.174±0.023、0.192±0.014 和 0.210±0.062。在第 14 天，与未加载对照相比，在 10、100 和 200ng/ml 组中，茜素红 S 染色的诺丽果值明显更高，100ng/ml 时最高。基于这些发现，我们得出结论，诺丽果提取物可能适用于增强干细胞球的成骨分化。
