Shrestha Abhishek, Grimm Maja, Ojiro Ichie, Krumwiede Johannes, Schikora Adam
Institute for Epidemiology and Pathogen Diagnostics, Federal Research Centre for Cultivated Plants, Julius Kühn-Institut, Braunschweig, Germany.
Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.
Front Microbiol. 2020 Jul 16;11:1545. doi: 10.3389/fmicb.2020.01545. eCollection 2020.
Bacterial quorum-sensing (QS) molecules are one of the primary means allowing communication between bacterial cells or populations. Plants also evolved to perceive and respond to those molecules. -acyl homoserine lactones (AHL) are QS molecules, of which impact has been extensively studied in different plants. Most studies, however, assessed the interactions in a bilateral manner, a nature of interactions, which occurs rarely, if at all, in nature. Here, we investigated how responds to the presence of different single AHL molecules and their combinations. We assumed that this reflects the situation in the rhizosphere more accurately than the presence of a single AHL molecule. In order to assess those effects, we monitored the plant growth and defense responses as well as resistance to the plant pathogen pathovar (). Our results indicate that the complex interactions between multiple AHL and plants may have surprisingly similar outcomes. Individually, some of the AHL molecules positively influenced plant growth, while others induced the already known AHL-priming for induced resistance. Their combinations had a relatively low impact on the growth but seemed to induce resistance mechanisms. Very striking was the fact that all triple, the quadruple as well as the double combination(s) with long-chained AHL molecules increased the resistance to . These findings indicate that induced resistance against plant pathogens could be one of the major outcomes of an AHL perception. Taken together, we present here the first study on how plants respond to the complexity of bacterial quorum sensing.
细菌群体感应（QS）分子是细菌细胞或群体之间进行交流的主要方式之一。植物也进化出了感知并对这些分子做出反应的能力。N-酰基高丝氨酸内酯（AHL）是群体感应分子，其对不同植物的影响已得到广泛研究。然而，大多数研究是以双边方式评估相互作用的，而这种相互作用的性质在自然界中很少（如果有的话）出现。在这里，我们研究了植物如何对不同单一AHL分子及其组合的存在做出反应。我们认为，这比单一AHL分子的存在更准确地反映了根际的情况。为了评估这些影响，我们监测了植物的生长和防御反应以及对植物病原菌丁香假单胞菌番茄致病变种（Pst）的抗性。我们的结果表明，多种AHL与植物之间的复杂相互作用可能会产生惊人相似的结果。单独来看，一些AHL分子对植物生长有积极影响，而其他一些则诱导了已知的AHL引发的诱导抗性。它们的组合对生长的影响相对较小，但似乎诱导了抗性机制。非常引人注目的是，所有与长链AHL分子的三重、四重以及双重组合都增加了对Pst的抗性。这些发现表明，对植物病原菌的诱导抗性可能是AHL感知的主要结果之一。综上所述，我们在此展示了关于植物如何应对细菌群体感应复杂性的第一项研究。
