Kroh Andreas, Ivanova Vanina, Drescher Hannah, Andruszkow Julia, Longerich Thomas, Nolting Jochen, Eickhoff Roman, Heise D, Rheinwalt Karl P, Neumann Ulf P, Ulmer Florian T
Department of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, 52074 Aachen, Germany.
Department of Internal Medicine III, RWTH Aachen University Hospital, 52074 Aachen, Germany.
Gastroenterol Res Pract. 2020 Jul 13;2020:7347068. doi: 10.1155/2020/7347068. eCollection 2020.
A variety of dietary nonalcoholic steatohepatitis (NASH) mouse models are available, and choosing the appropriate mouse model is one of the most important steps in the design of NASH studies. In addition to the histopathological and metabolic findings of NASH, a sufficient mouse model should guarantee a robust clinical status and good animal welfare. Three different NASH diets, a high-fat diet (HFD60), a western diet (WD), and a cafeteria diet (CAFD), were fed for 12 or 16 weeks. Metabolic assessment was conducted at baseline and before scheduled sacrifice, and liver inflammation was analyzed via fluorescence-associated cell sorting and histopathological examination. Clinical health conditions were scored weekly to assess the impact on animal welfare. The HFD60 and WD were identified as suitable NASH mouse models without a significant strain on animal welfare. Furthermore, the progression of inflammation and liver fibrosis was associated with a decreased proportion of CD3 NK1.1 cells. The WD represents a model of advanced-stage NASH, and the HFD60 is a strong model of nonalcoholic fatty liver disease (NAFLD) and metabolic syndrome. However, the CAFD should not be considered a NASH model.
有多种饮食诱导的非酒精性脂肪性肝炎（NASH）小鼠模型可供使用，选择合适的小鼠模型是NASH研究设计中最重要的步骤之一。除了NASH的组织病理学和代谢结果外，一个充分的小鼠模型应保证良好的临床状态和动物福利。给小鼠喂食三种不同的NASH饮食，即高脂饮食（HFD60）、西方饮食（WD）和自助餐厅饮食（CAFD），持续12周或16周。在基线和预定处死前进行代谢评估，并通过荧光相关细胞分选和组织病理学检查分析肝脏炎症。每周对临床健康状况进行评分，以评估对动物福利的影响。HFD60和WD被确定为合适的NASH小鼠模型，对动物福利没有显著影响。此外，炎症和肝纤维化的进展与CD3 NK1.1细胞比例的降低有关。WD代表晚期NASH模型，HFD60是一种强大的非酒精性脂肪性肝病（NAFLD）和代谢综合征模型。然而，CAFD不应被视为NASH模型。
