Xing Lei, Hong Xiaodong, Chang Liang, Ren Ping, Zhang Hong
Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Exp Ther Med. 2020 Aug;20(2):1637-1643. doi: 10.3892/etm.2020.8857. Epub 2020 Jun 10.
Non-small cell lung cancer (NSCLC) is a type of cancer that is associated with high prevalence and high mortality rates in China. Therefore, it is of importance to identify the mechanisms underlying NSCLC progression. In the present study, reverse transcription-quantitative PCR was performed to measure the expression of microRNA (miR)-365b in NSCLC cell lines. In addition, the biological roles of miR-365b and N-acetylgalactosaminyltransferase 4 (GALNT4) were investigated by manipulating the expression levels of miR-365b and GALNT4 in NSCLC cells. It was found that miR-365b expression was reduced in NSCLC tissues and cells. Overexpression of miR-365b inhibited NSCLC cell proliferation whilst promoting apoptosis, but miR-365b knockdown promoted NSCLC cell proliferation. In addition, it was demonstrated that miR-365b regulated the proliferation and apoptosis of NSCLC cells by targeting GALNT4 expression. Collectively, the present study identified a miR-365b/GALNT4 regulatory axis in NSCLC, suggesting that miR-365b may serve as a therapeutic target for NSCLC.
非小细胞肺癌（NSCLC）是一种在中国具有高发病率和高死亡率的癌症类型。因此，确定NSCLC进展的潜在机制具有重要意义。在本研究中，进行了逆转录定量PCR以检测非小细胞肺癌细胞系中微小RNA（miR）-365b的表达。此外，通过调控NSCLC细胞中miR-365b和N-乙酰半乳糖胺基转移酶4（GALNT4）的表达水平，研究了miR-365b和GALNT4的生物学作用。研究发现，NSCLC组织和细胞中miR-365b表达降低。miR-365b的过表达抑制NSCLC细胞增殖，同时促进细胞凋亡，但敲低miR-365b则促进NSCLC细胞增殖。此外，研究表明miR-365b通过靶向GALNT4表达来调节NSCLC细胞的增殖和凋亡。总体而言，本研究确定了NSCLC中的miR-365b/GALNT4调控轴，提示miR-365b可能作为NSCLC的治疗靶点。
