Ma Jing, Sun Siwen, Song Chen, Li Ning, Li Na, Xu Lingzhi, Yang Ting, Lan Yulong, Li Man
Department of Oncology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
Department of Foreign Languages, Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
Exp Ther Med. 2020 Sep;20(3):2705-2715. doi: 10.3892/etm.2020.8991. Epub 2020 Jul 13.
Pancreatic cancer is a malignant tumor of the digestive tract, rendering it difficult to make an accurate diagnosis. The 5 year survival rate for pancreatic cancer is <1%, and surgical resection rarely proves to be effective. Therefore, the identification of more effective methods for the early detection of pancreatic cancer is an urgent requirement. The present study aimed to explore key genes and microRNAs (miRNAs) associated with the pathogenesis of pancreatic cancer. Public databases were searched, and the data were integrated from The Cancer Genome Atlas and Gene Expression Omnibus databases, leading to the identification of 23 differentially expressed miRNAs (DE-miRNAs). A total of four of the DE-miRNAs were upregulated (hsa-miR-892b, hsa-miR-194-2, hsa-miR-200a and hsa-miR-194-1), whereas 19 downregulated DE-miRNAs (hsa-miR-424, hsa-miR-191, hsa-miR-484, hsa-miR-142, hsa-miR-15b, hsa-miR-450a-1, hsa-miR-423, hsa-miR-126, hsa-miR-505, hsa-miR-16-1, hsa-miR-342, hsa-miR-130a, hsa-miR-3613, hsa-miR-450a-2, hsa-miR-26b, hsa-miR-451, hsa-miR-19b-2, hsa-miR-106a and hsa-miR-503) were identified using the cut-off criteria of P<0.05 and |log 2FC|>1.0. Hsa-miR-3613-5p was identified as a prognostic DE-miRNA. The functional enrichment analyses demonstrated that the target genes of hsa-miR-3613-5p may be associated with the p53 signaling pathway. Survival analysis performed for genes in the p53 signaling pathway revealed that cyclin-dependent kinase 6 and ribonucleoside-diphosphate reductase subunit M2 may be the most likely to be associated with prognostic value. The integrated analysis performed in the current study demonstrated that hsa-miR-3613-5p may be used as a potential prognostic marker for pancreatic cancer.
胰腺癌是一种消化道恶性肿瘤，难以进行准确诊断。胰腺癌的5年生存率<1%，手术切除很少被证明是有效的。因此，寻找更有效的早期检测胰腺癌的方法迫在眉睫。本研究旨在探索与胰腺癌发病机制相关的关键基因和微小RNA（miRNA）。检索了公共数据库，并整合了来自癌症基因组图谱和基因表达综合数据库的数据，从而鉴定出23个差异表达的miRNA（DE-miRNA）。使用P<0.05和|log 2FC|>1.0的截断标准，共鉴定出4个上调的DE-miRNA（hsa-miR-892b、hsa-miR-194-2、hsa-miR-200a和hsa-miR-194-1），而19个下调的DE-miRNA（hsa-miR-424、hsa-miR-191、hsa-miR-484、hsa-miR-142、hsa-miR-15b、hsa-miR-450a-1、hsa-miR-423、hsa-miR-126、hsa-miR-505、hsa-miR-16-1、hsa-miR-342、hsa-miR-13o、hsa-miR-3613、hsa-miR-450a-2、hsa-miR-26b、hsa-miR-451、hsa-miR-19b-2、hsa-miR-106a和hsa-miR-503）。hsa-miR-3613-5p被鉴定为一种具有预后意义的DE-miRNA。功能富集分析表明，hsa-miR-3613-5p的靶基因可能与p53信号通路相关。对p53信号通路中的基因进行生存分析发现，细胞周期蛋白依赖性激酶6和核糖核苷二磷酸还原酶亚基M2最有可能与预后价值相关。本研究进行的综合分析表明，hsa-miR-3613-5p可能作为胰腺癌潜在的预后标志物。
