Department of Three Wards of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
Bioengineered. 2021 Dec;12(1):4633-4642. doi: 10.1080/21655979.2021.1956404.
In recent years, the increasing incidence of pancreatic carcinoma (PC) patients has become one of the hot issues in the world. microRNAs (miRNAs) can act as oncogenes or tumor suppressor genes and have unpredictable effects on tumors, thus affecting the prognosis and survival of cancer patients. In this paper, we mainly studied the role of microRNA (miR)-409 in PC. The expression levels of miR-409 were analyzed by qRT-PCR. Kaplan-Meier curve and Cox regression were used to analyze the relationship between miR-409 and patient prognosis. The effects of miR-409 on the abilities of proliferation, migration and invasion were detected by CCK-8 and Transwell. The expression levels of miR-409 were down-regulated in PC, compared with normal controls. The prognosis of patients with low miR-409 expression is significantly poor in comparison with those with high expression. The down-regulation of miR-409 was conducive to the proliferation, migration and invasion of PC cells. miR-409 is a tumor suppressor of PC, the clinical significance of miR-409 in pancreatic cancer and related tumor cell function was clarified.
近年来，胰腺癌（PC）患者的发病率不断上升，已成为全球关注的热点问题之一。微小 RNA（miRNA）可作为癌基因或抑癌基因，对肿瘤产生不可预测的影响，从而影响癌症患者的预后和生存。本文主要研究了 microRNA（miR）-409 在 PC 中的作用。通过 qRT-PCR 分析 miR-409 的表达水平。采用 Kaplan-Meier 曲线和 Cox 回归分析 miR-409 与患者预后的关系。通过 CCK-8 和 Transwell 检测 miR-409 对增殖、迁移和侵袭能力的影响。与正常对照组相比，PC 中 miR-409 的表达水平下调。与高表达相比，miR-409 低表达患者的预后明显较差。miR-409 的下调有利于 PC 细胞的增殖、迁移和侵袭。miR-409 是 PC 的肿瘤抑制因子，阐明了 miR-409 在胰腺癌及相关肿瘤细胞功能中的临床意义。
