长链非编码 RNA TUG1 过表达促进心肌细胞凋亡，并预测心肌梗死的预后不良。
LncRNA TUG1 overexpression promotes apoptosis of cardiomyocytes and predicts poor prognosis of myocardial infarction.
机构信息
Department of Cardiac Macrovascular Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
Department of Medical Imaging, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
出版信息
J Clin Pharm Ther. 2020 Dec;45(6):1452-1456. doi: 10.1111/jcpt.13190. Epub 2020 Aug 7.
WHAT IS KNOWN AND OBJECTIVE
Long non-coding RNAs (lncRNAs) have been proved to regulate. LncRNA TUG1 is well characterized in cancer biology. This study was carried out to investigate the molecular function of lncRNA TUG1 in myocardial infarction.
METHODS
This study enrolled 58 MI patients and 52 healthy volunteers, and blood samples were obtained from these participants. The expression levels of lncRNA TUG1 in plasma of MI patients and healthy volunteers were detected by qPCR. Overexpression experiments were performed to evaluate the interactions between lncRNA TUG1 and caspase 3.
RESULTS
The expression levels of lncRNA TUG1 were significantly increased in MI patients. And low lncRNA TUG1 expression group had a significantly higher overall survival rate than that of high lncRNA TUG1 expression group. Overexpression of lncRNA TUG1 promoted the apoptosis of AC16 cells and upregulated the expression of cleaved caspase- 3.
WHAT IS NEW AND RESULTS
Our study first reported the upregulation of lncRNA TUG1 in MI. Our results demonstrated that upregulation of lncRNA TUG1 effectively distinguished MI patients from the healthy controls. Therefore, upregulation of lncRNA TUG1 might serve as a potential diagnostic marker for MI.
已知和目的
长链非编码 RNA（lncRNAs）已被证明可以调节。lncRNA TUG1 在癌症生物学中得到了很好的描述。本研究旨在研究 lncRNA TUG1 在心肌梗死中的分子功能。
方法
本研究纳入了 58 例 MI 患者和 52 名健康志愿者，并从这些参与者中采集了血液样本。通过 qPCR 检测 MI 患者和健康志愿者血浆中 lncRNA TUG1 的表达水平。进行过表达实验以评估 lncRNA TUG1 与 caspase 3 之间的相互作用。
结果
lncRNA TUG1 在 MI 患者中的表达水平显著升高。低 lncRNA TUG1 表达组的总生存率明显高于高 lncRNA TUG1 表达组。lncRNA TUG1 的过表达促进了 AC16 细胞的凋亡，并上调了 cleaved caspase-3 的表达。
新发现和结果
我们的研究首次报道了 lncRNA TUG1 在 MI 中的上调。我们的结果表明，lncRNA TUG1 的上调有效地区分了 MI 患者和健康对照者。因此，lncRNA TUG1 的上调可能成为 MI 的潜在诊断标志物。
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