沉默 lncRNA 93358 通过诱导 SLC8A1 的表达抑制心肌梗死大鼠心肌细胞凋亡。
Silencing lncRNA 93358 Inhibits the Apoptosis of Myocardial Cells in Myocardial Infarction Rats by Inducing the Expression of SLC8A1.
机构信息
The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.
Department of Cardiology, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, China.
出版信息
Biomed Res Int. 2022 Jul 7;2022:1138709. doi: 10.1155/2022/1138709. eCollection 2022.
OBJECTIVE
To explore the inhibitor effects and mechanism of lncRNA 93358 against the apoptosis of myocardial cells in rats with myocardial infarction.
METHODS
The myocardial infarction model was established in rats, which were identified by cardiac ultrasound. TTC staining was used to evaluate the degree of heart infarction, and HE staining was utilized to determine the pathological state in myocardial tissues. The apoptotic state in myocardial tissues was confirmed by TUNEL assay. lncRNA 93358 was screened out using a high-throughput sequencing which was confirmed by RT-qPCR. The interaction between miR-466c-3p and SLC8A1 was identified using the dual-luciferase reporter assay. The expression level of Bax, Bcl-2, and SLC8A1 was determined in lncRNA 93358 knockdown cells using RT-qPCR and Western blotting.
RESULTS
Massive myocardial necrosis was observed in model rats according to the results of TTC staining, HE staining, and TUNEL assay. lncRNA 93358 and Bax were found significantly upregulated, and Bcl-2 and SLC8A1 were greatly downregulated in model rats, which were dramatically reversed by the knockdown of lncRNA 93358, accompanied by the decline area of myocardial necrosis and decreased apoptotic myocardial cells.
CONCLUSION
Silencing lncRNA 93358 inhibits the apoptosis of myocardial cells in rats with myocardial infarction by inducing the expression of SLC8A1.
目的
探讨长链非编码 RNA 93358 对心肌梗死后大鼠心肌细胞凋亡的抑制作用及机制。
方法
建立大鼠心肌梗死模型，通过心脏超声进行鉴定。TTC 染色评估心肌梗死程度，HE 染色确定心肌组织的病理状态。TUNEL 检测确定心肌组织的凋亡状态。通过高通量测序筛选出 lncRNA 93358，并用 RT-qPCR 进行验证。双荧光素酶报告实验鉴定 miR-466c-3p 与 SLC8A1 的相互作用。用 RT-qPCR 和 Western blot 检测 lncRNA 93358 敲低细胞中 Bax、Bcl-2 和 SLC8A1 的表达水平。
结果
TTC 染色、HE 染色和 TUNEL 检测结果显示模型大鼠存在大量心肌坏死。lncRNA 93358 和 Bax 表达显著上调，Bcl-2 和 SLC8A1 表达显著下调，lncRNA 93358 敲低可显著逆转这些变化，同时伴有心肌坏死面积减小和凋亡心肌细胞减少。
结论
沉默 lncRNA 93358 通过诱导 SLC8A1 的表达抑制心肌梗死后大鼠心肌细胞凋亡。
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