Tange T, Ahmed-Ansari A, Hansen T, Tse H Y
Department of Immunology, Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065.
J Immunol. 1988 Feb 1;140(3):700-4.
To test whether the major histocompatibility complex class I genes are involved in the regulation of hemopoiesis, the stem cell activities of BALB/c-H-2dm2 (Dm2) mice, which are defective in the expression of H-2L antigens, have been compared with those of the wild-type, BALB/c-Kh, in in vivo and in vitro stem cell assays. In spleen colony-forming unit assays, Dm2 as hosts consistently supported a smaller number of colonies than did BALB/c-Kh. However, both Dm2 and BALB/c-Kh supported a comparable number of colonies in in vitro granulocyte-macrophage colony-forming unit and erythroid colony-forming unit assays. These observations together suggest that the mutation in Dm2 has not affected the hemopoietic potential of the stem cells but may probably affect the hemopoietic microenvironment for the development of the stem cells.
为了检测主要组织相容性复合体I类基因是否参与造血调控，在体内和体外干细胞检测中，对H-2L抗原表达有缺陷的BALB/c-H-2dm2（Dm2）小鼠的干细胞活性与野生型BALB/c-Kh小鼠的干细胞活性进行了比较。在脾集落形成单位检测中，作为宿主的Dm2 consistently支持的集落数量比BALB/c-Kh少。然而，在体外粒细胞-巨噬细胞集落形成单位和红系集落形成单位检测中，Dm2和BALB/c-Kh支持的集落数量相当。这些观察结果共同表明，Dm2中的突变并未影响干细胞的造血潜能，但可能影响干细胞发育的造血微环境。
