Rath S, Stanley C M, Steward M W
Department of Medical Microbiology, London School of Hygiene and Tropical Medicine, U.K.
J Immunol Methods. 1988 Feb 10;106(2):245-9. doi: 10.1016/0022-1759(88)90204-9.
A method to measure the relative affinity of antibodies using an inhibition enzyme immunoassay is described. It is validated using monoclonal antibodies of defined affinity characteristics and by comparison with conventional methods of affinity measurement. The method allows measurement of the relative affinity of low levels of antibody, and the calculation of an empirical estimate of the heterogeneity of affinity in antibody populations.
描述了一种使用抑制酶免疫测定法测量抗体相对亲和力的方法。该方法通过具有明确亲和力特征的单克隆抗体进行验证，并与传统的亲和力测量方法进行比较。该方法能够测量低水平抗体的相对亲和力，并计算抗体群体中亲和力异质性的经验估计值。
