Fischer Luisa, Möller Palau-Ribes Franca, Enderlein Dirk, Fischer Dominik, Herbst Werner, Baudler Liane, Hafez Hafez Mohamed, Lierz Michael
Clinic for Birds, Reptiles, Amphibians and Fish, Justus Liebig University, Giessen, Germany.
Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University, Giessen, Germany.
Vet Microbiol. 2020 Aug;247:108789. doi: 10.1016/j.vetmic.2020.108789. Epub 2020 Jul 6.
The Mycoplasma strain ARNO was isolated from the semen of a clinically healthy gyrfalcon (Falco rusticolus). Colonies of strain ARNO grew in fried-egg shape on Mycoplasma agar plates (SP4). The organism did not ferment glucose or hydrolyze arginine or urea; hence, organic acids are assumed as energy source. Growth was sterol-dependent and optimal growth temperature 42 °C, with a temperature range from 20 to 44 °C. Strain ARNO was not identified as a representative of any of the currently described Mycoplasma species by alignment of the 16S rRNA gene sequence and 16 S-23 S intergenic transcribed spacer region, or immunobinding assay. Hence, strain ARNO represents a novel Mycoplasma species for which the name Mycoplasma seminis sp. nov. is proposed (DSM 27653, NCTC 13927). After developing a species-specific PCR, the prevalence of M. seminis sp. nov. was determined in adult and juvenile falcons in a commercial breeding center for falcons. Semen samples (n = 171) were obtained from 113 male adults, due to repeated sampling of 39 birds. Female adults (n = 26) were sampled once, while 105 of the 152 juvenile birds were sampled twice via choanal swabs. Mycoplasma seminis sp. nov. was found in the semen of clinically healthy adult males (3.5 %) as well as in the respiratory tract of female (34.6 %) and juvenile birds (59.2 %). After comparison of semen samples with (2.9 %) and without M. seminis sp. nov. identification, no indications for a potential influence on the semen quality were demonstrated. Hence, M. seminis sp. nov. seems likely to be of commensal character in falcons.
支原体菌株ARNO是从一只临床健康矛隼（矛隼）的精液中分离出来的。ARNO菌株的菌落以煎蛋形状生长在支原体琼脂平板（SP4）上。该生物体不发酵葡萄糖、不水解精氨酸或尿素；因此，假定有机酸为能量来源。生长依赖于固醇，最适生长温度为42℃，温度范围为20至44℃。通过16S rRNA基因序列和16S - 23S基因间转录间隔区的比对或免疫结合试验，ARNO菌株未被鉴定为任何目前已描述的支原体物种的代表。因此，ARNO菌株代表了一种新型支原体物种，为此提出了新种名精液支原体（Mycoplasma seminis sp. nov.）（DSM 27653，NCTC 13927）。在开发出一种物种特异性PCR后，在一个商业矛隼繁殖中心测定了成年和幼年矛隼中精液支原体新种的流行率。由于对39只鸟进行了重复采样，从113只成年雄性鸟中获得了171份精液样本。成年雌性鸟（n = 26）采样一次，而152只幼鸟中的105只通过后鼻孔拭子采样两次。在临床健康的成年雄性鸟的精液中（3.5%）以及雌性（34.6%）和幼年鸟的呼吸道中（59.2%）发现了精液支原体新种。在比较了鉴定出和未鉴定出精液支原体新种的精液样本（2.9%）后，未显示出对精液质量有潜在影响的迹象。因此，精液支原体新种在矛隼中似乎可能具有共生特性。
