Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
Syst Appl Microbiol. 2022 Jan;45(1):126292. doi: 10.1016/j.syapm.2021.126292. Epub 2021 Dec 25.
Since 2006, a Mycoplasma species unidentifiable to the species level has been regularly isolated from the semen and prepuce of apparently healthy bulls, and occasionally from cattle displaying inflammatory disease of the genital tract. Seven of these Mycoplasma isolates were subjected to a comprehensive taxonomic study. The strains investigated grew well in modified Hayflick's medium and colonies on agar exhibited typical fried egg morphology and produced 'film and spots'. Transmission electron microscopy revealed a cell morphology characteristic of mycoplasmas with spherically shaped cells bounded by a bi-layered cell membrane. The strains studied neither produced acid from sugar carbon sources nor did hydrolyse arginine or urea, and genome annotation indicated that organic acids (pyruvate, lactate) are used as energy sources. Phylogenetic analyses of 16S rRNA gene sequences, the 16S-23S intergenic spacer region, and partial rpoB gene and protein sequences placed the strains within the Mycoplasma (M.) bovis cluster of the Hominis group with M. primatum, M. agalactiae, and M. bovis being their closest relatives. Genomic information including whole-genome similarity metrics (ANIb, ANIm, TETRA, dDDH, AAI) and phylogenomics, proteomic features revealed by matrix-assisted laser desorption ionization time of flight (MALDI-ToF) mass spectrometry as well as serological reactions and polar lipid profiling strongly indicated that the strains examined were representatives of a hitherto unclassified species of genus Mycoplasma, for which the name Mycoplasma tauri sp. nov. with type strain Zaradi2 (=ATCC BAA-1891 = DSM 22451) is proposed.
自 2006 年以来，一种无法鉴定到种水平的支原体物种经常从看似健康公牛的精液和包皮中分离出来，偶尔也从生殖道有炎症的牛中分离出来。对其中的 7 个支原体分离株进行了全面的分类学研究。所研究的菌株在改良的 Hayflick 培养基中生长良好，琼脂上的菌落呈典型的煎蛋状形态，并产生“膜和斑点”。透射电子显微镜显示出一种典型的支原体细胞形态，细胞呈球形，由双层细胞膜包围。所研究的菌株既不能从糖碳源中产生酸，也不能水解精氨酸或尿素，基因组注释表明有机酸（丙酮酸、乳酸）被用作能源。16S rRNA 基因序列、16S-23S 间隔区、部分 rpoB 基因和蛋白序列的系统发育分析将这些菌株置于牛支原体（M. bovis）属的 Hominis 组内，与 M. primatum、M. agalactiae 和 M. bovis 关系最密切。基因组信息包括全基因组相似性指标（ANIb、ANIm、TETRA、dDDH、AAI）和系统基因组学、基质辅助激光解吸电离飞行时间（MALDI-ToF）质谱揭示的蛋白质组特征，以及血清学反应和极性脂谱强烈表明，所检查的菌株是一个尚未分类的支原体属代表种，该种被命名为 Mycoplasma tauri sp. nov.，其模式株为 Zaradi2（=ATCC BAA-1891=DSM 22451）。
