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评估在突尼斯对无症状人群进行 SARS-CoV-2 分子检测时进行样本混合的效果。

Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia.

机构信息

Charles Nicolle Hospital, Laboratory of Microbiology, Virology unit, National Influenza and other Respiratory Viruses Center-Tunisia, Boulevard 9 Avril, Tunis 1006, Tunisia; University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES09, 1007, Tunis, Tunisia.

University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES09, 1007, Tunis, Tunisia.

出版信息

Diagn Microbiol Infect Dis. 2020 Nov;98(3):115125. doi: 10.1016/j.diagmicrobio.2020.115125. Epub 2020 Jul 5.

DOI:10.1016/j.diagmicrobio.2020.115125
PMID:32768876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7335417/
Abstract

The aim of this study is to test a pooling approach for the RT-PCR test to detect low viral loads of SARS-CoV-2. We found that a single positive specimen can still be detected in pools of up to 10. Each laboratory should conduct its own evaluation and validation of pooling protocols according to its specific context.

摘要

本研究旨在测试 RT-PCR 检测低病毒载量 SARS-CoV-2 的合并检测方法。我们发现，在 10 个样本合并检测时，仍能检测到单个阳性样本。各实验室应根据其具体情况，自行评估和验证合并检测方案。

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PLoS One. 2020 Nov 4;15(11):e0240652. doi: 10.1371/journal.pone.0240652. eCollection 2020.
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Pooling of samples for testing for SARS-CoV-2 in asymptomatic people.对无症状人群进行新冠病毒检测的样本合并
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Evaluation of COVID-19 RT-qPCR Test in Multi sample Pools.评价多份样本混合的 COVID-19 RT-qPCR 检测。
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Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples.采用实时 RT-PCR 对常规呼吸道样本 RNA 制备的 mini 池进行 SARS-CoV-2 的临时基于实验室的监测。
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Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources.评估标本汇集以节约 SARS-CoV-2 检测资源。
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