Shi Jiangcheng, Hu Chengqing, Zhou Yuan, Cui Chunmei, Yang Jichun, Cui Qinghua
Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China.
Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China.
Mol Ther Nucleic Acids. 2020 Sep 4;21:687-695. doi: 10.1016/j.omtn.2020.07.006. Epub 2020 Jul 10.
Physiological and pathophysiological differences widely exist in paired organ systems. However, the molecular basis for these differences remains largely unknown. We previously reported that there exist differentially expressed miRNAs (DEMs) in the left and right kidneys of normal mice. Here, we identified the DEMs in the left and right eyes, lungs, and testes of normal mice via RNA sequencing. As a result, we identified 26 DEMs in eyes, with 23 higher and 3 lower in the left eyes compared with right eyes; 21 DEMs in lungs, with 15 higher and 6 lower in the left lungs compared with right lungs; and 54 DEMs in testes, with 6 higher and 48 lower in the left testes compared with right testes. Ten microRNAs (miRNAs) were further examined by quantitative PCR assays, and seven of these were confirmed. In addition, correlation analysis was performed between paired organ miRNA expressions and diverse body fluid miRNA expressions. Finally, we explored the functions and networks of DEMs and performed biological process and pathway enrichment analysis of target genes for DEMs, providing insights into the physiological and pathophysiological differences between the two entities of paired organs.
成对的器官系统中广泛存在生理和病理生理差异。然而，这些差异的分子基础在很大程度上仍然未知。我们之前报道过正常小鼠的左肾和右肾中存在差异表达的微小RNA（DEM）。在此，我们通过RNA测序鉴定了正常小鼠左眼和右眼、肺和睾丸中的DEM。结果，我们在眼中鉴定出26个DEM，与右眼相比，左眼有23个表达更高，3个表达更低；在肺中鉴定出21个DEM，与右肺相比，左肺有15个表达更高，6个表达更低；在睾丸中鉴定出54个DEM，与右睾丸相比，左睾丸有6个表达更高，48个表达更低。通过定量PCR分析进一步检测了10个微小RNA（miRNA），其中7个得到了证实。此外，还对成对器官miRNA表达与多种体液miRNA表达之间进行了相关性分析。最后，我们探索了DEM的功能和网络，并对DEM的靶基因进行了生物学过程和通路富集分析，为成对器官两个实体之间的生理和病理生理差异提供了见解。
