Department of Biomedical Informatics, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, MOE Key Laboratory of Molecular Cardiovascular Sciences, Center for Non-coding RNA Medicine, Peking University, Beijing, China.
Beijing Key Laboratory of Diabetes Research and Care, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
FEBS Lett. 2020 Feb;594(4):636-645. doi: 10.1002/1873-3468.13644. Epub 2019 Nov 14.
Physiological and pathophysiological differences exist between the left and right kidneys; however, the molecular bases for these differences remain unknown. Since miRNAs are involved in kidney function and the development of kidney diseases, we examined their differential expression through miRNA profiling of the left and right kidneys of normal mice. We find that 36 miRNAs exhibit higher expression, whereas 22 miRNAs show lower expression in the left than the right kidneys in mice under physiological condition. Ten miRNAs were further examined by quantitative PCR assays, and four of them with high expression level were confirmed by Northern blot. Through bioinformatic analysis, we dissected the function and network of the differentially expressed miRNAs, providing insights into the physiological and pathophysiological differences between the left and the right kidneys.
左右肾脏之间存在生理和病理生理学差异；然而，这些差异的分子基础尚不清楚。由于 miRNAs 参与肾脏功能和肾脏疾病的发生，我们通过对正常小鼠左右肾脏的 miRNA 谱进行分析，来检测它们的差异表达。我们发现，在生理条件下，36 个 miRNA 在左肾中表达较高，而 22 个 miRNA 在左肾中表达较低。通过定量 PCR 检测，进一步对其中 10 个 miRNA 进行了验证，其中 4 个 miRNA 用 Northern blot 进行了验证。通过生物信息学分析，我们对差异表达的 miRNA 的功能和网络进行了剖析，为左右肾脏的生理和病理生理学差异提供了深入了解。
