Nemoto R, Uchida K, Hattori K, Shimazui T, Nishijima Y, Saito S, Koiso K, Harada M
Department of Urology, University of Tsukuba, Ibaraki, Japan.
J Urol. 1988 Feb;139(2):286-9. doi: 10.1016/s0022-5347(17)42388-3.
A total of 18 patients with transitional cell bladder cancer was given a 0.5-hour intravenous infusion of bromodeoxyuridine at the time of endoscopic biopsy or transurethral resection to label tumor cells in the deoxyribonucleic acid synthesis phase (S phase). The tumor specimens were fixed with 70 per cent ethanol, embedded in paraffin, sectioned and stained by an indirect immunoperoxidase method with anti-bromodeoxyuridine monoclonal antibody as the first antibody. The bromodeoxyuridine labeling index, S phase fraction, was determined by counting the number of bromodeoxyuridine-labeled cells in the tissue sections. All grade 1 tumors had an S phase fraction of lower than 10 per cent. The average S phase fractions for noninvasive (11 cases) and invasive (7) tumors were 9.8 and 20.0 per cent, respectively. Two distant metastatic bladder tumors showed an average S phase fraction of 25.3 and 30.0 per cent. Thus, transitional cell bladder cancers with an S phase fraction of greater than 10 per cent appears to grow faster and be more invasive more often than those with an S phase fraction of less than 10 per cent. The higher S phase fraction may indicate greater biological malignancy. Our preliminary results suggest that measurement of the bromodeoxyuridine labeling index in bladder tumors may be a new objective and quantitative assay of biological potential of individual tumors.
18例移行细胞膀胱癌患者在内镜活检或经尿道切除时接受了0.5小时的溴脱氧尿苷静脉输注，以标记处于脱氧核糖核酸合成期（S期）的肿瘤细胞。肿瘤标本用70%乙醇固定，石蜡包埋，切片并用抗溴脱氧尿苷单克隆抗体作为一抗的间接免疫过氧化物酶法染色。通过计数组织切片中溴脱氧尿苷标记的细胞数量来确定溴脱氧尿苷标记指数，即S期分数。所有1级肿瘤的S期分数均低于10%。非侵袭性肿瘤（11例）和侵袭性肿瘤（7例）的平均S期分数分别为9.8%和20.0%。两个远处转移的膀胱肿瘤的平均S期分数分别为25.3%和30.0%。因此，S期分数大于10%的移行细胞膀胱癌似乎比S期分数小于10%的肿瘤生长更快且更具侵袭性。较高的S期分数可能表明更高的生物学恶性程度。我们的初步结果表明，测量膀胱肿瘤中的溴脱氧尿苷标记指数可能是一种新的客观且定量的个体肿瘤生物学潜能检测方法。
