Ohyama C, Nakazumi H, Satoh M, Yoshikawa K, Hoshi S, Orikasa S, Hayashi T, Mituno S, Murakami F, Kurosu S
Department of Urology, Tohoku University School of Medicine.
Nihon Hinyokika Gakkai Zasshi. 1990 Apr;81(4):524-31. doi: 10.5980/jpnjurol1989.81.524.
At the time of operation, 10 patients with bladder tumors were given an intravenous infusion of bromodeoxyuridine (BrdU, 250 mg) to label the cells in the DNA synthesis phase (S-phase). The removed tumor specimens were stained immunohistologically using anti-BrdU monoclonal antibody. The percentage of BrdU-labeled cells (S-phase cells) were calculated for each specimen, and expressed as Labeling Index (LI). The average LI of G1, G2 and G3 tumors were 0.9%, 2.3% and 11.9%, respectively. LI was almost parallel to pathological grade. In cases of G2 tumor, LI was varied from 0.2 to 6.2%. The higher LI, the more invasive was the tumor. The patient with the highest LI (12.2%) showed lung metastasis postoperatively and died 6 months after operation. S-phase cells were scattered along the basal layer in G1 papillary tumors, and were observed also near the superficial layer in G2 tumors. In cases of non-papillary tumors, S-phase cells were observed irregularly in various layers. This method will give us useful information on evaluation for malignancy and cell kinetics of bladder tumor.
手术时，对10例膀胱肿瘤患者静脉输注溴脱氧尿苷（BrdU，250mg）以标记处于DNA合成期（S期）的细胞。切除的肿瘤标本用抗BrdU单克隆抗体进行免疫组织化学染色。计算每个标本中BrdU标记细胞（S期细胞）的百分比，并表示为标记指数（LI）。G1、G2和G3级肿瘤的平均LI分别为0.9%、2.3%和11.9%。LI几乎与病理分级平行。在G2级肿瘤病例中，LI在0.2%至6.2%之间变化。LI越高，肿瘤的侵袭性越强。LI最高（12.2%）的患者术后出现肺转移，术后6个月死亡。在G1级乳头状肿瘤中，S期细胞沿基底层散在分布，在G2级肿瘤中也可见于表层附近。在非乳头状肿瘤病例中，S期细胞在各层中不规则分布。该方法将为我们评估膀胱肿瘤的恶性程度和细胞动力学提供有用信息。
