Department of Environmental Engineering, Faculty of Engineering and Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen, 40002, Thailand.
Center of Excellence On Hazardous Substance Management (HSM), Bangkok, 10330, Thailand.
Sci Rep. 2020 Aug 7;10(1):13391. doi: 10.1038/s41598-020-70355-w.
The occurrence of manganese in groundwater causes coloured water and pipe rusting in water treatment systems. Consumption of manganese-contaminated water promotes neurotoxicity in humans and animals. Manganese-oxidizing bacteria were isolated from contaminated areas in Thailand for removing manganese from water. The selected bacterium was investigated for its removal kinetics and mechanism using synchrotron-based techniques. Among 21 isolates, Streptomyces violarus strain SBP1 (SBP1) was the best manganese-oxidizing bacterium. At a manganese concentration of 1 mg L, SBP1 achieved up to 46% removal. The isolate also successfully removed other metal and metalloid, such as iron (81%) and arsenic (38%). The manganese concentration played a role in manganese removal and bacterial growth. The observed self-substrate inhibition best fit with the Aiba model. Kinetic parameters estimated from the model, including a specific growth rate, half-velocity constant, and inhibitory constant, were 0.095 h, 0.453 mg L, and 37.975 mg L, respectively. The synchrotron-based techniques indicated that SBP1 removed manganese via combination of bio-oxidation (80%) and adsorption (20%). The study is the first report on biological manganese removal mechanism using synchrotron-based techniques. SBP1 effectively removed manganese under board range of manganese concentrations. This result showed the potential use of the isolate for treating manganese-contaminated water.
地下水的锰含量会导致水系统中出现有色水和管道生锈。人类和动物饮用受锰污染的水会导致神经毒性。研究人员从泰国受污染地区分离出锰氧化菌，用于去除水中的锰。本研究采用同步辐射技术研究了所选细菌的去除动力学和机制。在 21 个分离株中，链霉菌属的 SBP1（SBP1）是最好的锰氧化菌。在锰浓度为 1mg/L 时，SBP1 的去除率最高可达 46%。该分离株还成功去除了其他金属和类金属，如铁（81%）和砷（38%）。锰浓度对锰的去除和细菌生长都有影响。观察到的自底物抑制作用最符合 Aiba 模型。从模型中估计的动力学参数，包括比生长速率、半速度常数和抑制常数，分别为 0.095h、0.453mg/L 和 37.975mg/L。同步辐射技术表明，SBP1 通过生物氧化（80%）和吸附（20%）的组合去除锰。本研究首次利用同步辐射技术报道了生物去除锰的机制。SBP1 在锰浓度的实际范围内有效地去除了锰。这一结果表明，该分离株有潜力用于处理受锰污染的水。
