Department of Biology, Texas Woman's University, Denton, TX, 76204-5799, USA.
Genes Immun. 2020 Nov;21(5):301-310. doi: 10.1038/s41435-020-0108-6. Epub 2020 Aug 7.
Animals counter specific environmental challenges with a combination of broad and tailored host responses. One protein family enlisted in the innate immune response includes the saposin-like antimicrobial proteins. We investigated the expression of a Caenorhabditis elegans saposin-like gene, spp-9, in response to different stresses. spp-9 expression was detected in the intestine and six amphid neurons, including AWB and AWC. spp-9 expression is increased in response to starvation stress. In addition, we discovered pathogen-specific regulation of spp-9 that was not clearly demarcated by Gram nature of the bacterial challenge. Multiple molecular innate immune response pathways, including DBL-1/TGF-β-like, insulin-like, and p38/MAPK, regulate expression of spp-9. Our results suggest spp-9 is involved in targeted responses to a variety of abiotic and bacterial challenges that are coordinated by multiple signaling pathways.
动物通过广泛和针对性的宿主反应来应对特定的环境挑战。在先天免疫反应中，一个被招募的蛋白家族包括类脑苷脂酶抗菌蛋白。我们研究了秀丽隐杆线虫中一种类脑苷脂酶样基因 spp-9 对不同应激的表达情况。spp-9 在肠道和六个触角神经元（包括 AWB 和 AWC）中被检测到。spp-9 的表达在饥饿应激下增加。此外，我们发现 spp-9 的表达受到病原体的特异性调控，而这种调控并不完全由细菌挑战的革兰氏性质来划分。多种分子先天免疫反应途径，包括 DBL-1/TGF-β 样、胰岛素样和 p38/MAPK，调节 spp-9 的表达。我们的结果表明，spp-9 参与了针对多种非生物和细菌挑战的靶向反应，这些反应由多种信号通路协调。
