Konishi Keisuke, Du Lei, Francius Grégory, Linder Michel, Sugawara Tomoaki, Kurihara Hideyuki, Takahashi Koretaro
Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido, 041-8611, Japan.
Department of Nutrition and Food Hygiene, School of Public Health, Cheeloo College of Medicine, Shandong University, No.44 Wenhuaxi Road, Jinan, Shandong, 250012, China.
Lipids. 2020 Nov;55(6):671-682. doi: 10.1002/lipd.12269. Epub 2020 Aug 8.
Lipid composition of liposomal bilayer should alter the cell response for permeability, transport, and uptake in small intestine. This work was done to investigate the transport and uptake of liposomes composed of docosahexaenoic acid-enriched phosphatidylcholine (PtdCho), phosphatidylserine (PtdSer), and sulfoquinovosyl diacylglycerol (SQDG) derived from marine products on multilamellar vesicles (MLV) in small intestinal epithelial cell models. The results showed that addition of PtdSer and SQDG as liposomal bilayer could improve the efficiency entrapment of liposomes. The liposomes containing PtdSer showed higher transport and uptake through both Caco-2 cell and M cell monolayers as compared to PtdCho-MLV. SQDG-containing liposomes exhibited only higher transport through M cell monolayer, while its uptake effect was higher both in Caco-2 cell and M cell monolayers. The results of experiments done with endocytosis inhibitors indicated that PtdCho-MLV must be transported via macropinocytosis and uptaken by phagocytosis in M cell monolayer model. PtdCho/PtdSer-MLV and PtdCho/SQDG-MLV might be transported and uptaken through M cell monolayer by phagocytosis. The result also indicated that PtdCho/SQDG-MLV could open the tight junction of small intestinal epithelial cell monolayers. Furthermore, our findings demonstrated that the surface status of cholesterol-containing liposomes were smooth, but they did not affect their transport and uptake through Caco-2 cell and M cell monolayers.
脂质体双层的脂质组成应改变小肠中细胞对通透性、转运和摄取的反应。本研究旨在探讨由富含二十二碳六烯酸的磷脂酰胆碱（PtdCho）、磷脂酰丝氨酸（PtdSer）和源自海产品的磺基喹喔啉基二酰甘油（SQDG）组成的脂质体在小肠上皮细胞模型中对多层囊泡（MLV）的转运和摄取情况。结果表明，添加PtdSer和SQDG作为脂质体双层可提高脂质体的包封效率。与PtdCho-MLV相比，含有PtdSer的脂质体通过Caco-2细胞和M细胞单层的转运和摄取更高。含SQDG的脂质体仅通过M细胞单层表现出更高的转运，而其摄取效应在Caco-2细胞和M细胞单层中均更高。用内吞作用抑制剂进行的实验结果表明，PtdCho-MLV在M细胞单层模型中必须通过巨胞饮作用转运并通过吞噬作用摄取。PtdCho/PtdSer-MLV和PtdCho/SQDG-MLV可能通过吞噬作用通过M细胞单层转运和摄取。结果还表明，PtdCho/SQDG-MLV可以打开小肠上皮细胞单层的紧密连接。此外，我们的研究结果表明，含胆固醇的脂质体表面状态光滑，但它们不影响其通过Caco-2细胞和M细胞单层的转运和摄取。
