Grant Alice H, Terminel Mabel A, Ramos Jeremiah, Alatorre Luisa F, Castañeda Edward
Department of Psychology, University of Texas at El Paso, 500 W. University Ave, El Paso, TX, USA.
Department of Psychology, University of Texas at El Paso, 500 W. University Ave, El Paso, TX, USA; Department of Psychology, Arizona State University, Tempe, AZ, USA.
J Neurosci Methods. 2020 Dec 1;346:108894. doi: 10.1016/j.jneumeth.2020.108894. Epub 2020 Aug 6.
Electrical Stimulation is a traditional tool in neuroscience and is commonly used in vivo to evoke behavior and in vitro to study neural mechanisms. In vivo intracerebral microdialysis, also a traditional technique, is used to assay neurotransmitter release. However, the combination of these techniques is highly limited to studies using anesthetized animals; therefore, evoking and measuring exocytotic neurotransmitter release in awake models is lacking. Combining these techniques in an awake animal preparation is presented here with evidence to support the mechanistic action of electrical stimulation in vivo.
This report presents converging evidence to validate the combination of intracerebral electrical stimulation with microdialysis as a novel procedure to study exocytotic-like dopamine release in behaving animals.
It is shown that electrical stimulation of the medial forebrain bundle can be used to evoke frequency- and intensity-dependent exocytotic-like dopamine overflow and rotational behavior that are sensitive to Na channel blockade and Ca availability.
Studies using modern techniques to evoke neurotransmitter release, combined with in vivo intracerebral microdialysis, and measured behavioral output are scarce. In contrast, commonly used pharmacological methods often are less precise and inefficient to evoke exocytotic dopamine release and behavior. Here we demonstrate, the combination of in vivo intracerebral microdialysis with electrical stimulation as a simple approach to simultaneously assess physiologically relevant neurotransmitter 'release' and behavior.
Research that aims to understand how dopamine neurotransmission is altered in behavioral disorders can utilize this innovative combination of electrical stimulation with in vivo intracerebral microdialysis.
电刺激是神经科学中的一种传统工具，常用于体内诱发行为以及体外研究神经机制。体内脑微透析也是一种传统技术，用于测定神经递质释放。然而，这些技术的结合在很大程度上仅限于对麻醉动物的研究；因此，缺乏在清醒模型中诱发和测量胞吐性神经递质释放的方法。本文介绍了在清醒动物制备中结合这些技术的方法，并提供了支持电刺激在体内作用机制的证据。
本报告提供了一系列证据，以验证脑内电刺激与微透析相结合作为一种研究行为动物中类似胞吐性多巴胺释放的新方法。
结果表明，内侧前脑束的电刺激可用于诱发频率和强度依赖性的类似胞吐性多巴胺溢出以及对钠通道阻断和钙可用性敏感的旋转行为。
使用现代技术诱发神经递质释放并结合体内脑微透析以及测量行为输出的研究很少。相比之下，常用的药理学方法在诱发胞吐性多巴胺释放和行为方面往往不太精确且效率低下。在这里，我们证明了体内脑微透析与电刺激相结合是一种同时评估生理相关神经递质“释放”和行为的简单方法。
旨在了解行为障碍中多巴胺神经传递如何改变的研究可以利用电刺激与体内脑微透析这种创新的结合方法。
