Seed Science and Technology Lab, College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China.
Seed Science and Technology Lab, College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China.
Plant Physiol Biochem. 2020 Sep;154:751-757. doi: 10.1016/j.plaphy.2020.05.042. Epub 2020 Jun 6.
Reactive oxygen species (ROS) are increasingly recognized as non-enzymatic players in the processes of radicle elongation growth and endosperm weakening during seed germination. NADPH oxidases (EC 1.6.3.1), also known as respiratory burst oxidase homologues (Rbohs), are key enzymes for the production of ROS. We previously reported that ROS are involved in the radicle elongation growth and endosperm weakening during lettuce seed germination. However, the function of the gene(s) encoding Rbohs during lettuce seed germination remains to be elucidated. In this study, one lettuce Rboh gene LsRbohC1 was cloned, and over-expression and RNAi-lines of this gene were generated. It was found that LsRbohC1 was abundantly expressed in germinating seeds, especially in the endosperm cap and hypocotyl. Over-expression/knock-down of this gene observably increased/decreased the production of superoxide radicals in the radicle and endosperm cap, and significantly promoted/delayed the germination process. The results suggest that LsRbohC1 plays a role in lettuce seed germination.
活性氧（ROS）在种子萌发过程中的胚根伸长生长和胚乳弱化中越来越被认为是非酶促因子。NADPH 氧化酶（EC 1.6.3.1），也称为呼吸爆发氧化酶同源物（Rbohs），是产生 ROS 的关键酶。我们之前报道过 ROS 参与了生菜种子萌发过程中的胚根伸长生长和胚乳弱化。然而，编码 Rbohs 的基因（s）在生菜种子萌发过程中的功能仍有待阐明。在这项研究中，克隆了一个生菜 Rboh 基因 LsRbohC1，并生成了该基因的过表达和 RNAi 系。发现 LsRbohC1 在萌发的种子中大量表达，特别是在胚乳帽和下胚轴中。该基因的过表达/敲低显著增加/减少了胚根和胚乳帽中超氧自由基的产生，并显著促进/延迟了萌发过程。结果表明，LsRbohC1 在生菜种子萌发中起作用。
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