Department of Laboratory Medicine, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Shenyang 110004, China.
Department of Laboratory Medicine, The Sixth People's Hospital of Shenyang, No. 85 Heping South Street, Shenyang 110006, China.
Int Immunopharmacol. 2020 Nov;88:106861. doi: 10.1016/j.intimp.2020.106861. Epub 2020 Jul 30.
Corona Virus Disease 2019 (COVID-19) has spread rapidly to more than 215 countries, with over 11.91 million reported cases and more than 540,000 deaths. Rapid diagnosis remains a bottleneck for containing the epidemic. We used an automated chemiluminescent immunoassay to detect serum IgM and IgG antibodies to the 2019-nCoV in 742 subjects, so as to observe the dynamic process of antibody production in COVID-19 disease and seroepidemiology in different populations. Patients with COVID-19 were reactive (positive) for specific antibodies within 3-15 days after onset of symptoms. Specific IgM and IgG levels increased with the progression of the disease. The areas under the receiver operating characteristic curves for IgM and IgG were 0.984 and 1.000, respectively. This antibody detection assay had good sensitivity and specificity. The understanding of the dynamic serological changes of COVID-19 patients and the seroepidemiological situation of the population will be helpful to further control the epidemic of COVID-19.
2019 年冠状病毒病（COVID-19）迅速蔓延至 215 个以上国家，报告病例超过 1191 万例，死亡超过 54 万例。快速诊断仍然是控制疫情的瓶颈。我们使用自动化化学发光免疫分析检测了 742 例患者血清中 2019 年新型冠状病毒的 IgM 和 IgG 抗体，以观察 COVID-19 疾病中抗体产生的动态过程和不同人群中的血清流行病学。COVID-19 患者在症状出现后 3-15 天内对特异性抗体呈反应（阳性）。特异性 IgM 和 IgG 水平随疾病的进展而增加。IgM 和 IgG 的受试者工作特征曲线下面积分别为 0.984 和 1.000。该抗体检测分析具有良好的灵敏度和特异性。了解 COVID-19 患者的动态血清学变化和人群的血清流行病学情况，将有助于进一步控制 COVID-19 的流行。
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