Physiology Section, Department of Cell Biology, Physiology and Immunology, Faculty of Biology, Universitat de Barcelona, Barcelona, Spain.
Laboratory of Exercise Physiology, Department of Health Science, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.
Eur J Appl Physiol. 2020 Oct;120(10):2339-2348. doi: 10.1007/s00421-020-04456-9. Epub 2020 Aug 9.
The aerobic exercise affects the respiratory redox-state. The influence of different relative humidity (RH) levels on the formation of respiratory reactive chemical species associated with redox-state altered by exercise has been poorly explored. Our aim was to evaluate the effect of two different RH conditions (40% vs. 90%) on the concentration of hydrogen peroxide and nitrite in exhaled breath condensate ([HO] and [NO]) and spirometry parameters in recreational cyclists.
Sixteen men and women (12/4) (mean age ± SD: 23.5 ± 2.2 years) completed 60-min of cycling at 166.3 ± 26.9 watts (70% of maximum load of [Formula: see text]-max. test, 49.3 ± 7.6 mL·min·kg) at random 40%-RH and 90%-RH conditions separated by 7 days. The two-way RM-ANOVA test was applied to compare [HO], [NO], [NO]/[NO] at rest and 80-min post-exercise (80-post); and spirometry parameters at rest, 20-post and 80-post.
The interaction of factors (humidity × time) was significant in [HO], [NO], [NO]/[NO] (p = 0.005, p = 0.030, p = 0.043, respectively). At 40%-RH conditions, the same parameters were higher in 80-post than at rest (p < 0.001, p = 0.001, p = 0.014, respectively). At the same time, the [HO] and [NO]/[NO] were higher in 40%-RH than 90%-RH (p = 0.010, p < 0.001, respectively). The interaction was significant in FEV (p = 0.013) and FEF (p = 0.023), but not in FEV/FVC (p = 0.362). At 80-post, the changes are kept in 90%-RH (p < 0.001), diminishing in 40%-RH being similar to rest.
In recreational cyclists, 90%-RH prevents the increase of hydrogen peroxide and nitrite in exhaled breath condensate samples observed at 40%-RH and prolonging the bronchodilation until 80-post cycling exercise.
有氧运动影响呼吸的氧化还原状态。不同相对湿度（RH）水平对运动引起的氧化还原状态改变相关的呼吸活性化学物质形成的影响尚未得到充分探讨。我们的目的是评估两种不同 RH 条件（40%对 90%）对运动后 80 分钟时（80-post）呼气冷凝物（[HO]和[NO]）中过氧化氢和亚硝酸盐浓度以及休闲自行车运动员用力肺活量（FEV）参数的影响。
16 名男性和女性（12/4）（平均年龄 ± 标准差：23.5 ± 2.2 岁）以 166.3 ± 26.9 瓦（70%最大负荷测试的[Formula: see text]-max. 测试，49.3 ± 7.6 mL·min·kg）随机在 40% RH 和 90% RH 条件下各进行 60 分钟的踏车运动，两种 RH 条件之间间隔 7 天。采用双向 RM-ANOVA 检验比较休息时和运动后 80 分钟（80-post）时的[HO]、[NO]、[NO]/[NO]以及休息时、运动后 20 分钟（20-post）和 80 分钟（80-post）时的用力肺活量参数。
湿度 × 时间的交互作用在[HO]、[NO]、[NO]/[NO]方面均有显著性（p = 0.005、p = 0.030、p = 0.043，分别）。在 40% RH 条件下，与休息时相比，80-post 时的相同参数均升高（p < 0.001、p = 0.001、p = 0.014，分别）。同时，[HO]和[NO]/[NO]在 40% RH 时均高于 90% RH（p = 0.010、p < 0.001，分别）。FEV（p = 0.013）和 FEF（p = 0.023）的交互作用具有显著性，但 FEV/FVC（p = 0.362）的交互作用无显著性。在 80-post 时，90% RH 时的变化保持不变（p < 0.001），而 40% RH 时的变化减少，与休息时相似。
在休闲自行车运动员中，90% RH 可防止在 40% RH 时观察到的呼气冷凝物样本中过氧化氢和亚硝酸盐浓度的增加，并将支气管扩张作用延长至运动后 80 分钟。
