State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
Arch Virol. 2020 Nov;165(11):2479-2486. doi: 10.1007/s00705-020-04739-8. Epub 2020 Aug 9.
Prunus necrotic ringspot virus (PNRSV) is a viral pathogen with worldwide distribution, infecting many commercial fruit trees and ornamental plants. So far, the correlation between PNRSV infection and China rose mosaic disease has not been studied. Rose mosaic disease is characterized by severe symptoms, including mosaic, line pattern, and ringspot. Six viruses that were potentially associated with mosaic disease, including PNRSV, were tested in China roses. Only PNRSV was detected in China roses showing mosaic disease, and asymptomatic samples tested negative for this virus. This result was confirmed by small RNA sequencing, but rose leaf rosette-associated virus and rose spring dwarf-associated virus were also identified in both samples with mosaic disease and asymptomatic samples. This implied that PNRSV might be associated with China rose mosaic disease. Full genome sequences of two PNRSV isolates were determined, and the RNA1, 2 and 3 segments were found to be 3,332, 2,594 and 1,951 nucleotides (nt) in length, respectively. The three RNA segments shared 88.7-89.1% nt sequence identity in the 3'UTR, while RNA2 and RNA3 shared 98.2-99.4% identity. The higher variability in RNA1 suggests that it might have been under greater selection pressure. Phylogenetic analysis showed that the two PNRSV isolates clustered in group PV-32. Full-length infectious cDNA clones of PNRSV from China rose were constructed and used to agroinfiltrate cucumber seedlings. The inoculated cucumber leaves showed yellowing, chlorotic spots, necrosis, dwarfing, and decline at 23 to 39 days post-inoculation, demonstrating the virulence of the PNRSV isolate from China rose. These data lay a foundation for determining the molecular mechanism of rose mosaic disease caused by PNRSV.
李坏死环斑病毒（PNRSV）是一种具有全球分布的病毒病原体，感染许多商业果树和观赏植物。到目前为止，PNRSV 感染与中国玫瑰花叶病之间的相关性尚未得到研究。玫瑰花叶病的特征是症状严重，包括花叶、线条图案和环斑。对可能与花叶病相关的 6 种病毒，包括 PNRSV，在中国玫瑰中进行了测试。只有在表现花叶病的中国玫瑰中检测到 PNRSV，而无症状样本对此病毒检测为阴性。这一结果通过小 RNA 测序得到了证实，但在花叶病和无症状样本中也鉴定出了玫瑰叶卷曲相关病毒和玫瑰春季矮化相关病毒。这表明 PNRSV 可能与中国玫瑰花叶病有关。两种 PNRSV 分离株的全长基因组序列被测定，RNA1、2 和 3 片段的长度分别为 3332、2594 和 1951 个核苷酸（nt）。三个 RNA 片段在 3'UTR 中具有 88.7-89.1%的 nt 序列同一性，而 RNA2 和 RNA3 则具有 98.2-99.4%的同一性。RNA1 的更高变异性表明它可能受到更大的选择压力。系统进化分析表明，两个 PNRSV 分离株聚集在 PV-32 组中。构建了来自中国玫瑰的 PNRSV 的全长感染性 cDNA 克隆，并用于 agroinfiltration 黄瓜幼苗。接种的黄瓜叶片在 23 至 39 天的接种后出现变黄、黄化斑点、坏死、矮化和衰退，表明中国玫瑰 PNRSV 分离株的毒力。这些数据为确定 PNRSV 引起的玫瑰花叶病的分子机制奠定了基础。
