Department of Pathology and Laboratory Medicine, Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY.
Appl Immunohistochem Mol Morphol. 2020 Nov/Dec;28(10):791-793. doi: 10.1097/PAI.0000000000000832.
The importance of technical quality for histopathologic examination has only increased in recent years with the expanding use of digital pathology. The University of Kentucky Alzheimer's Disease Center (UK-ADC) Neuropathology Core has decades of experience with brain histopathology and has emphasized the importance of quantitative assessments of histopathologic hallmarks. Technical artifacts and nonuniform samples are challenging for high-throughput digital analyses after the slides have been scanned, so that methodological optimization may be helpful. We do not know of published literature that systematically reviews how different procedures at the various stages of tissue processing can impact the quality of the histopathologic preparations in human brain samples. We wanted to pass along our experience in the hope that it will help others to improve their results. Here we describe the UK-ADC method of embedding for neuropathologic evaluation and provide specific examples (with a comparison to another processing workflow) that help support the idea that the methods and tools used in the embedding process can alter the quality of the formalin-fixed paraffin-embedded histopathologic results. The process used at the UK-ADC has been successful for us, but results may vary in relation to each embedding machine and with other factors.
近年来，随着数字病理学的广泛应用，组织病理学检查的技术质量变得尤为重要。肯塔基大学阿尔茨海默病中心（UK-ADC）神经病理学核心拥有数十年的脑组织病理学经验，并强调了对组织病理学特征进行定量评估的重要性。在扫描载玻片后，技术伪影和非均匀样本对高通量数字分析构成了挑战，因此可能需要进行方法优化。我们不知道有任何已发表的文献系统地回顾了组织处理的各个阶段的不同步骤如何影响人脑样本中组织病理学准备的质量。我们希望分享我们的经验，希望能帮助其他人改善他们的结果。在这里，我们描述了 UK-ADC 用于神经病理学评估的包埋方法，并提供了具体示例（与另一种处理工作流程进行比较），这些示例有助于支持这样一种观点，即包埋过程中使用的方法和工具可以改变福尔马林固定石蜡包埋组织病理学结果的质量。在 UK-ADC 中使用的方法对我们来说是成功的，但结果可能因每个包埋机和其他因素而异。
