Trautz Florian, Dreßler Jan, Stassart Ruth, Müller Wolf, Ondruschka Benjamin
Institute of Legal Medicine, Medical Faculty, University of Leipzig, Leipzig, Germany.
Department of Neuropathology, University Hospital, University of Leipzig, Leipzig, Germany.
Int J Legal Med. 2018 Jul;132(4):1103-1109. doi: 10.1007/s00414-017-1767-3. Epub 2018 Jan 3.
Immunohistochemistry (IHC) has become an integral part in forensic histopathology over the last decades. However, the underlying methods for IHC vary greatly depending on the institution, creating a lack of comparability. The aim of this study was to assess the optimal approach for different technical aspects of IHC, in order to improve and standardize this procedure. Therefore, qualitative results from manual and automatic IHC staining of brain samples were compared, as well as potential differences in suitability of common IHC glass slides. Further, possibilities of image digitalization and connected issues were investigated. In our study, automatic staining showed more consistent staining results, compared to manual staining procedures. Digitalization and digital post-processing facilitated direct analysis and analysis for reproducibility considerably. No differences were found for different commercially available microscopic glass slides regarding suitability of IHC brain researches, but a certain rate of tissue loss should be expected during the staining process.
在过去几十年里，免疫组织化学（IHC）已成为法医组织病理学中不可或缺的一部分。然而，免疫组织化学的基础方法因机构不同而有很大差异，导致缺乏可比性。本研究的目的是评估免疫组织化学不同技术方面的最佳方法，以改进和规范这一程序。因此，比较了脑样本手动和自动免疫组织化学染色的定性结果，以及常见免疫组织化学载玻片适用性的潜在差异。此外，还研究了图像数字化的可能性及相关问题。在我们的研究中，与手动染色程序相比，自动染色显示出更一致的染色结果。数字化和数字后处理极大地促进了直接分析和可重复性分析。对于免疫组织化学脑研究的适用性，不同市售显微镜载玻片未发现差异，但在染色过程中应预期会有一定比例的组织损失。
