Allen Rachael S, Bales Katie, Feola Andrew, Pardue Machelle T
Center of Excellence for Visual and Neurocognitive Rehabilitation, Atlanta Veterans Affairs Medical Center; Department of Biomedical Engineering, Georgia Institute of Technology;
Center of Excellence for Visual and Neurocognitive Rehabilitation, Atlanta Veterans Affairs Medical Center; Department of Ophthalmology, Emory University.
J Vis Exp. 2020 Jul 24(161). doi: 10.3791/61588.
Spectral-domain optical coherence tomography (SD-OCT) is useful for visualizing retinal and ocular structures in vivo. In research, SD-OCT is a valuable tool to evaluate and characterize changes in a variety of retinal and ocular disease and injury models. In light induced retinal degeneration models, SD-OCT can be used to track thinning of the photoreceptor layer over time. In glaucoma models, SD-OCT can be used to monitor decreased retinal nerve fiber layer and total retinal thickness and to observe optic nerve cupping after inducing ocular hypertension. In diabetic rodents, SD-OCT has helped researchers observe decreased total retinal thickness as well as decreased thickness of specific retinal layers, particularly the retinal nerve fiber layer with disease progression. In mouse models of myopia, SD-OCT can be used to evaluate axial parameters, such as axial length changes. Advantages of SD-OCT include in vivo imaging of ocular structures, the ability to quantitatively track changes in ocular dimensions over time, and its rapid scanning speed and high resolution. Here, we detail the methods of SD-OCT and show examples of its use in our laboratory in models of retinal degeneration, glaucoma, diabetic retinopathy, and myopia. Methods include anesthesia, SD-OCT imaging, and processing of the images for thickness measurements.
光谱域光学相干断层扫描（SD-OCT）有助于在体内可视化视网膜和眼部结构。在研究中，SD-OCT是评估和表征各种视网膜和眼部疾病及损伤模型变化的宝贵工具。在光诱导视网膜变性模型中，SD-OCT可用于随时间追踪光感受器层的变薄情况。在青光眼模型中，SD-OCT可用于监测视网膜神经纤维层厚度和视网膜总厚度的降低，并在诱导高眼压后观察视神经杯状凹陷。在糖尿病啮齿动物中，SD-OCT帮助研究人员观察到随着疾病进展视网膜总厚度以及特定视网膜层厚度的降低，尤其是视网膜神经纤维层。在近视小鼠模型中，SD-OCT可用于评估轴向参数，如眼轴长度变化。SD-OCT的优点包括眼部结构的体内成像、随时间定量追踪眼部尺寸变化的能力，以及其快速扫描速度和高分辨率。在此，我们详细介绍SD-OCT的方法，并展示其在我们实验室用于视网膜变性、青光眼、糖尿病视网膜病变和近视模型的应用实例。方法包括麻醉、SD-OCT成像以及用于厚度测量的图像处理。
