Tanrıverdi Osman, Yılmaz İlhan, Erdoğan Uzay, Günaldı Ömür, Güngör Abuzer, Adilay Hüseyin Utku, Arslanhan Ayça, Tuğcu Bekir
Departments of Neurosurgery and Psychiatry, Bakırköy Dr. Mazhar Osman Mental Health and Neurological Diseases Training and Research Hospital, Istanbul, Turkey.
Department of Neurosurgery, University of Health Sciences, Sisli Hamidiye Etfal Training and Research Hospital, Istanbul, Turkey.
Sisli Etfal Hastan Tip Bul. 2018 Dec 28;52(4):268-273. doi: 10.14744/SEMB.2018.35761. eCollection 2018.
The aim of this study was to determine if Manuka honey, a potent anti-inflammatory and antioxidant agent, had any effect on the development of vasospasm in an experimental subarachnoidal hemorrhage model constructed in rat femoral arteries.
Twenty-four Wistar Albino strain rats were divided into 3 groups: Group 1 was the control group (n=8), Group 2 was the vasospasm group (n=8), and group 3 was the treatment group (n=8). The wall thickness (W) of the femoral arteries and the luminal diameter (L) were measured using morphometric methods. The data were analyzed with statistical software. The Mann-Whitney U-test was used to compare independent groups and Bonferroni post hoc analysis was used for multiple comparison tests. Significance for all of the results was established at p<0.05.
A statistically significant intergroup difference was detected in the mean L and W (p<0.001, p=0.001, respectively). The mean L value in Group 2 was statistically significantly less than that of Groups 1 and 3, while the mean W value was significantly greater (p<0.001 for all). However, no statistically significant difference was detected between Groups 1 and 3 with respect to the mean L and W values (p=0.064, p=0.954, respectively).
Manuka honey exerts an antioxidant and anti-inflammatory effect via inhibition of inflammatory cytokines, including plasma tumor necrosis factor alpha, interleukin (IL)-1 beta, IL-6, and the lipid peroxidation level. This study statistically demonstrated that the anti-inflammatory and antioxidant properties of Manuka honey successfully inhibited the development of vasospasm in an experimentally induced vasospasm model in the femoral arteries of rats.
本研究旨在确定麦卢卡蜂蜜（一种有效的抗炎和抗氧化剂）对在大鼠股动脉构建的实验性蛛网膜下腔出血模型中血管痉挛的发展是否有任何影响。
将24只Wistar白化病品系大鼠分为3组：第1组为对照组（n = 8），第2组为血管痉挛组（n = 8），第3组为治疗组（n = 8）。使用形态测量方法测量股动脉的壁厚（W）和管腔直径（L）。用统计软件分析数据。采用Mann-Whitney U检验比较独立组，采用Bonferroni事后分析进行多重比较检验。所有结果的显著性设定为p<0.05。
在平均L和W中检测到组间有统计学显著差异（分别为p<0.001，p = 0.001）。第2组的平均L值在统计学上显著低于第1组和第3组，而平均W值显著更高（所有p<0.001）。然而，在平均L和W值方面，第1组和第3组之间未检测到统计学显著差异（分别为p = 0.064，p = 0.954）。
麦卢卡蜂蜜通过抑制炎性细胞因子，包括血浆肿瘤坏死因子α、白细胞介素（IL）-1β、IL-6以及脂质过氧化水平，发挥抗氧化和抗炎作用。本研究从统计学上证明，麦卢卡蜂蜜的抗炎和抗氧化特性成功抑制了大鼠股动脉实验性诱导血管痉挛模型中血管痉挛的发展。
