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绘制鸡灯刷染色体上的表观遗传修饰图谱。

Mapping epigenetic modifications on chicken lampbrush chromosomes.

作者信息

Kulikova Tatiana, Surkova Anna, Zlotina Anna, Krasikova Alla

机构信息

Saint Petersburg State University, Saint-Petersburg, Russia.

出版信息

Mol Cytogenet. 2020 Aug 3;13:32. doi: 10.1186/s13039-020-00496-0. eCollection 2020.

DOI:10.1186/s13039-020-00496-0
PMID:32774459
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7397634/
Abstract

BACKGROUND

The epigenetic regulation of genome is crucial for implementation of the genetic program of ontogenesis through establishing and maintaining differential gene expression. Thus mapping of various epigenetic modifications to the genome is relevant for studying the regulation of gene expression. Giant transcriptionally active lampbrush chromosomes are an established tool for high resolution physical mapping of the genome and its epigenetic modifications. This study is aimed at characterizing the epigenetic status of compact chromatin domains (chromomeres) of chicken lampbrush macrochromosomes.

RESULTS

Distribution of three epigenetic modifications - 5-methylcytosine, histone H3 trimethylated at lysine 9 and hyperacetylated histone H4 - along the axes of chicken lampbrush chromosomes 1-4, Z and W was analyzed in details. Enrichment of chromatin domains with the investigated epigenetic modifications was indicated on the cytological chromomere-loop maps for corresponding chicken lampbrush chromosomes. Heterogeneity in the distribution of 5-methylcytosine and histone H3 trimethylated at lysine 9 along the chromosome axes was revealed.

CONCLUSIONS

On examples of certain chromomeres of chicken lampbrush chromosomes 1, 3, 4 and W we demonstrated that a combination of immunofluorescent staining and fluorescence in situ hybridization allows to relate the epigenetic status and a DNA sequence context of individual chromomeres.

摘要

背景

基因组的表观遗传调控对于通过建立和维持基因表达差异来实现个体发育的遗传程序至关重要。因此，将各种表观遗传修饰定位到基因组上与研究基因表达调控相关。巨大的转录活跃灯刷染色体是用于基因组及其表观遗传修饰的高分辨率物理图谱绘制的既定工具。本研究旨在表征鸡灯刷大染色体的紧密染色质结构域（染色粒）的表观遗传状态。

结果

详细分析了三种表观遗传修饰——5-甲基胞嘧啶、赖氨酸9位点三甲基化的组蛋白H3和高乙酰化组蛋白H4——在鸡灯刷染色体1-4、Z和W轴上的分布。在相应鸡灯刷染色体的细胞学染色粒-袢环图谱上显示了具有所研究表观遗传修饰的染色质结构域的富集情况。揭示了5-甲基胞嘧啶和赖氨酸9位点三甲基化的组蛋白H3在染色体轴上分布的异质性。

结论

以鸡灯刷染色体1、3、4和W的某些染色粒为例，我们证明免疫荧光染色和荧光原位杂交相结合能够将单个染色粒的表观遗传状态与DNA序列背景联系起来。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/fdaa06307e7c/13039_2020_496_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/13337fd3d520/13039_2020_496_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/21eef41f8a72/13039_2020_496_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/9a1a92690ddc/13039_2020_496_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/fbf97961a99c/13039_2020_496_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/f5d045f731f5/13039_2020_496_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/fdaa06307e7c/13039_2020_496_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/13337fd3d520/13039_2020_496_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/f1e8b0acdf40/13039_2020_496_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/21eef41f8a72/13039_2020_496_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/9a1a92690ddc/13039_2020_496_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/fbf97961a99c/13039_2020_496_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/f5d045f731f5/13039_2020_496_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6e4/7397634/fdaa06307e7c/13039_2020_496_Fig7_HTML.jpg

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Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes.鸡灯刷染色体的单细胞 DNA 甲基化分析。
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本文引用的文献

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Front Genet. 2020 Feb 17;11:57. doi: 10.3389/fgene.2020.00057. eCollection 2020.
2
3D organization of chicken genome demonstrates evolutionary conservation of topologically associated domains and highlights unique architecture of erythrocytes' chromatin.鸡基因组的三维结构展示了拓扑相关结构域的进化保守性，并突出了红细胞染色质的独特结构。
Nucleic Acids Res. 2019 Jan 25;47(2):648-665. doi: 10.1093/nar/gky1103.
3
New high copy tandem repeat in the content of the chicken W chromosome.
转录活跃的减数分裂 I 前期卵母细胞中体 TAD 与灯刷染色体环复合物的比较。
Chromosoma. 2022 Dec;131(4):207-223. doi: 10.1007/s00412-022-00780-5. Epub 2022 Aug 29.
鸡W染色体中出现新的高拷贝串联重复序列。
Chromosoma. 2018 Mar;127(1):73-83. doi: 10.1007/s00412-017-0646-5. Epub 2017 Sep 26.
4
Chicken Microchromosomes in the Lampbrush Phase: A Cytogenetic Description.灯刷期鸡的微小染色体：细胞遗传学描述
Cytogenet Genome Res. 2017;152(1):46-54. doi: 10.1159/000475563. Epub 2017 Jun 1.
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Avian W and mammalian Y chromosomes convergently retained dosage-sensitive regulators.鸟类的W染色体和哺乳动物的Y染色体趋同地保留了剂量敏感调节因子。
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