Wang Xianwang, Wang Pengli, Ge Lei, Wang Juan, Naqvi Syed Manzar Abbas Shah, Hu Shujuan
Department of Biochemistry and Molecular Biology, Health Science Center, Yangtze University, Jingzhou, Hubei 434023, P.R. China.
Laboratory of Oncology, Center for Molecular Medicine, Health Science Center, Yangtze University, Jingzhou, Hubei 434023, P.R. China.
Oncol Lett. 2020 Oct;20(4):12. doi: 10.3892/ol.2020.11873. Epub 2020 Jul 15.
Skin cutaneous melanoma (SKCM) is the most aggressive type of skin cancer, with a high rate of metastasis and mortality; however, identification of biomarkers for the treatment of SKCM is required. Cluster of differentiation (CD)38 has emerged as an effective target for therapeutic drugs in several types of cancer, such as chronic lymphocytic leukemia and multiple myeloma. In the present study, to determine the contribution of CD38 to the diagnosis of SKCM, Gene Expression Profiling Interactive Analysis 2 and University of Alabama Cancer Database online tools were used to analyze The Cancer Genome Atlas-SKCM dataset. Moreover, Search Tool for the Retrieval of Interacting Genes/Proteins and GeneMANIA databases were used to determine protein-protein interaction networks and potential functions. To the best of our knowledge, the results of the present study indicated for the first time that high expression levels of CD38 were a favorable diagnostic factor for SKCM. Moreover, a correlation between CD38 expression levels and the survival probability of patients with SKCM was identified. Integrative analysis predicted that nine genes were correlated with CD38 in SKCM, and the similarity of these genes in SKCM expression and a survival heatmap was verified. Gene ontology enrichment analysis using the Metascape tool revealed that CD38 and its correlated genes were significantly enriched in lymphocyte activation and T cell differentiation regulation. Collectively, the bioinformatics analysis revealed that CD38 might serve as a potential diagnostic predictor for SKCM.
皮肤黑色素瘤（SKCM）是最具侵袭性的皮肤癌类型，转移率和死亡率都很高；然而，需要鉴定用于治疗SKCM的生物标志物。分化簇（CD）38已成为几种癌症（如慢性淋巴细胞白血病和多发性骨髓瘤）治疗药物的有效靶点。在本研究中，为了确定CD38对SKCM诊断的贡献，使用基因表达谱交互分析2和阿拉巴马大学癌症数据库在线工具分析了癌症基因组图谱-SKCM数据集。此外，使用检索相互作用基因/蛋白质的搜索工具和GeneMANIA数据库来确定蛋白质-蛋白质相互作用网络和潜在功能。据我们所知，本研究结果首次表明CD38高表达水平是SKCM的有利诊断因素。此外，还确定了CD38表达水平与SKCM患者生存概率之间的相关性。综合分析预测，在SKCM中有9个基因与CD38相关，并验证了这些基因在SKCM表达中的相似性以及生存热图。使用Metascape工具进行的基因本体富集分析表明，CD38及其相关基因在淋巴细胞活化和T细胞分化调节中显著富集。总的来说，生物信息学分析表明CD38可能作为SKCM的潜在诊断预测指标。
