State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China.
College of Marine Science, Shandong University, Weihai 264209, PR China.
Int J Syst Evol Microbiol. 2020 Sep;70(9):4993-5000. doi: 10.1099/ijsem.0.004369.
Strain SDU3-2 was isolated from a soil sample collected in Shandong Province, PR China. Cells of SDU3-2 were spherical, Gram-stain-positive, aerobic and non-motile. Cellular growth of the strain occurred at 25-45 °C, pH 5.5-8.5 and with 0-1.5 % (w/v) of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain SDU3-2 was closest to the type strain ALT-1b with a similarity of 95.2 %. The draft genome was 3.49 Mbp long with 69.2 mol% G+C content. Strain SDU3-2 exhibited high resistance to gamma radiation (D >12 kGy) and UV (D >900 J m). The strain encoded many genes for resistance to radiation and oxidative stress, which were highly conserved with other species, but possessed interspecific properties. The major fatty acids of SDU3-2 cells were C 6, C 7/C 6, and C 8, the major menaquinone was menaquinone-8, and the major polar lipids were an unidentified phosphoglycolipid, four unidentified glycolipids and an unidentified phospholipid. The average nucleotide identity and DNA-DNA hybridization results further indicated that strain SDU3-2 represents a new species in the genus , for which the name sp. nov. is proposed. The type strain is SDU3-2 (=CGMCC 1.17147=KCTC 43098).
菌株 SDU3-2 从中国山东省采集的土壤样本中分离得到。SDU3-2 细胞呈球形，革兰氏阳性，需氧，不运动。该菌株的细胞生长发生在 25-45°C、pH5.5-8.5 和 0-1.5%（w/v）的 NaCl 下。基于 16S rRNA 基因序列的系统发育分析表明，菌株 SDU3-2 与模式菌株 ALT-1b 最为接近，相似度为 95.2%。基因组草图长 3.49 Mbp，GC 含量为 69.2%mol。菌株 SDU3-2 对伽马辐射（D >12 kGy）和紫外线（D >900 J m）具有高抗性。该菌株编码了许多对辐射和氧化应激有抗性的基因，这些基因与其他物种高度保守，但具有种间特性。SDU3-2 细胞的主要脂肪酸为 C6、C7/C6 和 C8，主要的menaquinone 为menaquinone-8，主要的极性脂为一种未知的磷酸甘油酯、四种未知的糖脂和一种未知的磷脂。平均核苷酸同一性和 DNA-DNA 杂交结果进一步表明，菌株 SDU3-2 代表了属中的一个新种，因此提议使用新种名 sp. nov.。模式菌株为 SDU3-2（=CGMCC 1.17147=KCTC 43098）。
