College of Horticulture, Shandong Agricultural Universities, Taian, Shandong, China.
Ministry of Agriculture Key Laboratory of Seaweed Fertilizers, Qingdao, China.
J Appl Microbiol. 2021 Mar;130(3):926-936. doi: 10.1111/jam.14818. Epub 2020 Aug 24.
Fusarium oxysporum is the primary pathogen causing root rot disease that severely affects cultivation of jujube fruit in the Xinjiang province of China. The aim of this study was to identify endophytic bacteria in healthy jujube organs that could effectively suppress F. oxysporum growth.
Different plant organs (leaves, twigs and roots) were collected from healthy Chinese jujube cultivated in southern Xinjiang province of China. The endophytic bacterium Brevibacterium halotolerans JZ7 was selected for its strong antagonistic activity and growth-promoting characteristics. Gas-chromatography mass-spectrometry analysis showed that acetoin, 2,3-butanediol and fenretinide were the three dominant volatile organic compounds produced by strain JZ7. Fenretinide strongly suppressed spore germination of F. oxysporum in vitro. Pot experiments showed that strain JZ7 colonized both the roots and rhizosphere soil of Chinese jujube and significantly reduced F. oxysporum level in jujube rhizosphere soil.
We demonstrated that B. halotolerans JZ7 can be developed into a biological control agent to combat root rot disease of Chinese jujube in the Xinjiang province of China.
The suggested strategy for biological control of jujube root rot disease is fully in accordance with the current principles of sustainability.
尖孢镰刀菌是导致中国新疆枣树根腐病的主要病原体。本研究旨在从健康的枣树器官中鉴定出能有效抑制尖孢镰刀菌生长的内生细菌。
从中国新疆南部地区种植的健康枣树不同器官（叶片、小枝和根）中采集内生细菌。选择具有强烈拮抗活性和促生长特性的耐盐短杆菌 JZ7 作为内生细菌。气相色谱-质谱分析表明，菌株 JZ7 产生的三种主要挥发性有机化合物为乙酰酮、2,3-丁二醇和芬维 A 醇。芬维 A 醇体外强烈抑制尖孢镰刀菌孢子萌发。盆栽试验表明，菌株 JZ7 能在枣树根系和根际土壤中定殖，并显著降低枣树根际土壤中尖孢镰刀菌的水平。
本研究表明，耐盐短杆菌 JZ7 可开发为防治中国新疆枣树根腐病的生物防治剂。
该研究提出的枣树根腐病生物防治策略完全符合可持续发展的当前原则。
