Guoyi Xu, Xiaolong Song, Yongli Ku, Yuan Tian, Ming Li, Cuiling Cao, Huili Yu, Peng Si
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, China.
Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang, Henan, China.
Front Microbiol. 2025 Aug 21;16:1623463. doi: 10.3389/fmicb.2025.1623463. eCollection 2025.
As the world's largest producer of kiwifruit, China faces significant yield and quality losses due to the widespread occurrence of kiwifruit root rot. To explore alternative biological control strategies for kiwifruit root rot, this study isolated 11 fungal isolates from diseased kiwifruit roots and identified as the primary pathogen. Additionally, a biocontrol strain, C3, was isolated from the rhizosphere of healthy kiwifruit and shown to significantly inhibit pathogen growth. The C3 strain effectively controls root rot via multiple mechanisms, including direct antagonism, secretion of antimicrobial proteins, promotion of seedling growth, and induction of plant defense enzymes. In pot and field trials, C3 treatment increased root fresh weight by 84.1%, enhanced root SOD and APX activities by 45.7 and 38.2%, respectively, and reduced disease severity. Moreover, C3 improved rhizosphere soil microbial diversity of the Rhizosphere, with the Shannon index increasing from 3.0 to 3.4. Unlike previous studies focusing solely on pathogen suppression, this work highlights the dual role of C3 in controlling root rot and restoring rhizosphere ecological function, offering a green and sustainable biocontrol strategy for kiwifruit production.
作为世界上最大的猕猴桃生产国，由于猕猴桃根腐病的广泛发生，中国面临着显著的产量和质量损失。为了探索猕猴桃根腐病的替代生物防治策略，本研究从患病猕猴桃根部分离出11株真菌菌株，并鉴定为主要病原菌。此外，从健康猕猴桃根际分离出一种生物防治菌株C3，它能显著抑制病原菌生长。C3菌株通过多种机制有效控制根腐病，包括直接拮抗、分泌抗菌蛋白、促进幼苗生长和诱导植物防御酶。在盆栽和田间试验中，C3处理使根鲜重增加了84.1%，根超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性分别提高了45.7%和38.2%，并降低了病害严重程度。此外，C3改善了根际土壤微生物多样性，香农指数从3.0提高到3.4。与以往仅关注病原菌抑制的研究不同，这项工作突出了C3在控制根腐病和恢复根际生态功能方面的双重作用，为猕猴桃生产提供了一种绿色可持续的生物防治策略。
